TY - JOUR
T1 - Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
AU - Crozet, Pierre
AU - Navarro, Francisco J.
AU - Willmund, Felix
AU - Mehrshahi, Payam
AU - Bakowski, Kamil
AU - Lauersen, Kyle J.
AU - Pérez-Pérez, Maria Esther
AU - Auroy, Pascaline
AU - Gorchs Rovira, Aleix
AU - Sauret-Gueto, Susana
AU - Niemeyer, Justus
AU - Spaniol, Benjamin
AU - Theis, Jasmine
AU - Trösch, Raphael
AU - Westrich, Lisa Desiree
AU - Vavitsas, Konstantinos
AU - Baier, Thomas
AU - Hübner, Wolfgang
AU - De Carpentier, Felix
AU - Cassarini, Mathieu
AU - Danon, Antoine
AU - Henri, Julien
AU - Marchand, Christophe H.
AU - De Mia, Marcello
AU - Sarkissian, Kevin
AU - Baulcombe, David C.
AU - Peltier, Gilles
AU - Crespo, José Luis
AU - Kruse, Olaf
AU - Jensen, Poul Erik
AU - Schroda, Michael
AU - Smith, Alison G.
AU - Lemaire, Stéphane D.
N1 - Generated from Scopus record by KAUST IRTS on 2019-11-20
PY - 2018/9/21
Y1 - 2018/9/21
N2 - Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.
AB - Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.
UR - https://pubs.acs.org/doi/10.1021/acssynbio.8b00251
UR - http://www.scopus.com/inward/record.url?scp=85053304568&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.8b00251
DO - 10.1021/acssynbio.8b00251
M3 - Article
C2 - 30165733
SN - 2161-5063
VL - 7
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 9
ER -