Bioformulation Containing Cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8 for the Nutrient Enhancement of Cicer arietinum L

Nitin Baliyan, Kamal A. Qureshi, Mariusz Jaremko, Minakshi Rajput, Monika Singh, Sandhya Dhiman, Dinesh Kumar Maheshwari, Chandra Kant, Ajay Kumar

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Here we examine the effects of different carrier based bioinoculants on the growth, yield and nutritional value of chickpea and on associated soil nutrients. A consortium of two taxonomically distinct endophytic bacteria—Ensifer adhaerens MSN12 and Bacillus cereus MEN8—have promising plant growth promoting (PGP) attributes. We demonstrate their delivery from the laboratory to the field via the formulation of an effective bioinoculant with economic and accessible carriers. Sugarcane straw ash (SCSA) was found to be an efficient carrier and bioformulation for enhancing viability and shelf-life of strains up to 12 months. A bioformulation containing an SCSA-based consortium (MSN12 + MEN8) increased seed germination by 7%, plant weight by 29%, length by 17%, seed-yield by 12%, harvesting index by 14% and proximate nutritional constituents by 20% over consortium treatment without SCSA. In addition, the bioformulation of post-harvest treated soil improved the physico-chemical properties of the soil in comparison to a pre-sowing SCSA-based bioformulation treated crop, being fortified in different proximate nutritional constituents including dry matter (30%), crude protein (45%), crude fiber (35%), and ether extract (40%) in comparison to the control. Principal component analysis and scattered matrix plots showed a positive correlation among the treatments, which also validates improvement in the soil nutrient components and proximate constituents by T6 treatment (MSN12 + MEN8 + SCSA). The above results suggest efficiency of SCSA not only as a carrier material but also to support microbial growth for adequate delivery of lab strains as a substitute for chemi-fertilizers.
Original languageEnglish (US)
Pages (from-to)3123
Issue number22
StatePublished - Nov 15 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-11-17
Acknowledgements: The A.P.C. was funded by the King Abdullah University of Science and Technology (KAUST), Thuwal, Jeddah, Saudi Arabia. The authors wish to thank the Head, Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar (India) for providing the necessary facilities.


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