Bioactive Steroids from the Red Sea Soft Coral $\textit{Sinularia polydactyla}$.

Mohamed A Tammam, Lucie Rárová, Marie Kvasnicová, Gabriel Gonzalez, Ahmed M Emam, Aldoushy Mahdy, Miroslav Strnad, Efstathia Ioannou, Vassilios Roussis

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Six new (1, 2, 6, 8, 13, and 20) and twenty previously isolated (3-5, 7, 9-12, 14-19, and 21-26) steroids featuring thirteen different carbocycle motifs were isolated from the organic extract of the soft coral $\textit{Sinularia polydactyla}$ collected from the Hurghada reef in the Red Sea. The structures and the relative configurations of the isolated natural products have been determined based on extensive analysis of their NMR and MS data. The cytotoxic, anti-inflammatory, anti-angiogenic, and neuroprotective activity of compounds 3-7, 9-12, 14-20, and 22-26, as well as their effect on androgen receptor-regulated transcription was evaluated in vitro in human tumor and non-cancerous cells. Steroids 22 and 23 showed significant cytotoxicity in the low micromolar range against the HeLa and MCF7 cancer cell lines, while migration of endothelial cells was inhibited by compounds 11, 12, 22, and 23 at 20 µM. The results of the androgen receptor (AR) reporter assay showed that compound 11 exhibited the strongest inhibition of AR at 10 µM, while it is noteworthy that steroids 10, 16, and 20 displayed increased inhibition of AR with decreasing concentrations. Additionally, compounds 11 and 23 showed neuroprotective activity on neuron-like SH-SY5Y cells.
Original languageEnglish (US)
Pages (from-to)632
JournalMarine Drugs
Volume18
Issue number12
DOIs
StatePublished - Dec 16 2020
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2021-09-09
Acknowledgements: M.A.T. acknowledges support by the mission sector of the Ministry of Higher Education of the Arab Republic of Egypt (Egyptian Cultural Bureau in Athens), the Directorate of Education and Cultural Affairs, Ministry of Foreign Affairs of Greece and the non-profit organization “Kleon Tsetis”. We thank Jitka Ulrichová for the kind gift of HUVEC cells and Zdeněk Dvořák for the kind gift of 22Rv1-ARE14 cells. Authors also thank Anežka Šindlerová and Veronika Górová for excellent technical assistance. Assistance on the acquisition of some NMR data by Lukasz Jaremko (King Abdullah University of Science and Technology, Saudi Arabia) is gratefully acknowledged.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

  • Drug Discovery

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