Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

Ricardo N. Alves; Maria Rambla-Alegre; Ana Catarina Braga; Ana L. Mauhrault; Vera Barbosa; Monica Campas; Laia Reverte; Cintia Flores; Josep Caixach; Jane Kilcoyne; Pedro Reis Costa; Jorge Diogene; Antonio Marque

doi:10.1016/j.fct.2019.04.041

Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

Ricardo N. Alves, Maria Rambla-Alegre, Ana Catarina Braga, Ana L. Mauhrault, Vera Barbosa, Monica Campas, Laia Reverte, Cintia Flores, Josep Caixach, Jane Kilcoyne, Pedro Reis Costa, Jorge Diogene, Antonio Marque

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid group ranged from 69% (raw European razor clams) to 74% (raw donax clams). Regarding azaspiracids, 47% of the initial content was bioaccessible in steamed blue mussel. As for hydrophilic toxins, 100% of the initial content was bioaccessible after digestion in raw shellfish and puffer fish gonads. The total tetrodotoxin bioaccessibility in puffer fish gonads decreased significantly after steaming. The profile of tetrodotoxins changed during the digestion process: TTX and 11-norTTX-6S-ol analogues decreased significantly after digestion, but the 5,6,11-trideoxy TTX analogue increased in both raw and steamed puffer fish gonads. These preliminary findings confirm the need to consider bioaccessibility data in future seafood risk assessment, as such information enables a more accurate and realistic estimation of potential seafood hazards, particularly in what concerns lipophilic toxins, therefore, constituting a crucial tool in the refinement of regulatory limits for the presence of biotoxins in seafood.

Original language	English (US)
Pages (from-to)	153-161
Number of pages	9
Journal	Food and Chemical Toxicology
Volume	129
DOIs	https://doi.org/10.1016/j.fct.2019.04.041
State	Published - 2019
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2021-07-12
Acknowledgements: The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and through the European Regional Development Fund. IRTA members acknowledge support from CERCA Programme/Generalitat de Catalunya. L.R. acknowledges a scholarship from the ECsafeSEAFOOD project. Ana C. Braga has a Doctoral Grant (PD/BD/113484/2015) from the Portuguese Science and Technology Foundation (FCT), whereas Antonio Marques and Pedro R. Costa are supported through the FCT Investigator program (IF). Lagocephalus sceleratus was kindly provided by Andrés Izquierdo-Muñoz from Centro de Investigación Marina de Santa Pola (CIMA) (Spain).

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Alves, R. N., Rambla-Alegre, M., Braga, A. C., Mauhrault, A. L., Barbosa, V., Campas, M., Reverte, L., Flores, C., Caixach, J., Kilcoyne, J., Costa, P. R., Diogene, J., & Marque, A. (2019). Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach. Food and Chemical Toxicology, 129, 153-161. https://doi.org/10.1016/j.fct.2019.04.041

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title = "Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach",

abstract = "This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid group ranged from 69% (raw European razor clams) to 74% (raw donax clams). Regarding azaspiracids, 47% of the initial content was bioaccessible in steamed blue mussel. As for hydrophilic toxins, 100% of the initial content was bioaccessible after digestion in raw shellfish and puffer fish gonads. The total tetrodotoxin bioaccessibility in puffer fish gonads decreased significantly after steaming. The profile of tetrodotoxins changed during the digestion process: TTX and 11-norTTX-6S-ol analogues decreased significantly after digestion, but the 5,6,11-trideoxy TTX analogue increased in both raw and steamed puffer fish gonads. These preliminary findings confirm the need to consider bioaccessibility data in future seafood risk assessment, as such information enables a more accurate and realistic estimation of potential seafood hazards, particularly in what concerns lipophilic toxins, therefore, constituting a crucial tool in the refinement of regulatory limits for the presence of biotoxins in seafood.",

author = "Alves, {Ricardo N.} and Maria Rambla-Alegre and Braga, {Ana Catarina} and Mauhrault, {Ana L.} and Vera Barbosa and Monica Campas and Laia Reverte and Cintia Flores and Josep Caixach and Jane Kilcoyne and Costa, {Pedro Reis} and Jorge Diogene and Antonio Marque",

note = "KAUST Repository Item: Exported on 2021-07-12 Acknowledgements: The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and through the European Regional Development Fund. IRTA members acknowledge support from CERCA Programme/Generalitat de Catalunya. L.R. acknowledges a scholarship from the ECsafeSEAFOOD project. Ana C. Braga has a Doctoral Grant (PD/BD/113484/2015) from the Portuguese Science and Technology Foundation (FCT), whereas Antonio Marques and Pedro R. Costa are supported through the FCT Investigator program (IF). Lagocephalus sceleratus was kindly provided by Andr{\'e}s Izquierdo-Mu{\~n}oz from Centro de Investigaci{\'o}n Marina de Santa Pola (CIMA) (Spain).",

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doi = "10.1016/j.fct.2019.04.041",

language = "English (US)",

volume = "129",

pages = "153--161",

journal = "Food and Chemical Toxicology",

issn = "1873-6351",

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Alves, RN, Rambla-Alegre, M, Braga, AC, Mauhrault, AL, Barbosa, V, Campas, M, Reverte, L, Flores, C, Caixach, J, Kilcoyne, J, Costa, PR, Diogene, J & Marque, A 2019, 'Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach', Food and Chemical Toxicology, vol. 129, pp. 153-161. https://doi.org/10.1016/j.fct.2019.04.041

TY - JOUR

T1 - Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

AU - Alves, Ricardo N.

AU - Rambla-Alegre, Maria

AU - Braga, Ana Catarina

AU - Mauhrault, Ana L.

AU - Barbosa, Vera

AU - Campas, Monica

AU - Reverte, Laia

AU - Flores, Cintia

AU - Caixach, Josep

AU - Kilcoyne, Jane

AU - Costa, Pedro Reis

AU - Diogene, Jorge

AU - Marque, Antonio

N1 - KAUST Repository Item: Exported on 2021-07-12 Acknowledgements: The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and through the European Regional Development Fund. IRTA members acknowledge support from CERCA Programme/Generalitat de Catalunya. L.R. acknowledges a scholarship from the ECsafeSEAFOOD project. Ana C. Braga has a Doctoral Grant (PD/BD/113484/2015) from the Portuguese Science and Technology Foundation (FCT), whereas Antonio Marques and Pedro R. Costa are supported through the FCT Investigator program (IF). Lagocephalus sceleratus was kindly provided by Andrés Izquierdo-Muñoz from Centro de Investigación Marina de Santa Pola (CIMA) (Spain).

PY - 2019

Y1 - 2019

N2 - This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid group ranged from 69% (raw European razor clams) to 74% (raw donax clams). Regarding azaspiracids, 47% of the initial content was bioaccessible in steamed blue mussel. As for hydrophilic toxins, 100% of the initial content was bioaccessible after digestion in raw shellfish and puffer fish gonads. The total tetrodotoxin bioaccessibility in puffer fish gonads decreased significantly after steaming. The profile of tetrodotoxins changed during the digestion process: TTX and 11-norTTX-6S-ol analogues decreased significantly after digestion, but the 5,6,11-trideoxy TTX analogue increased in both raw and steamed puffer fish gonads. These preliminary findings confirm the need to consider bioaccessibility data in future seafood risk assessment, as such information enables a more accurate and realistic estimation of potential seafood hazards, particularly in what concerns lipophilic toxins, therefore, constituting a crucial tool in the refinement of regulatory limits for the presence of biotoxins in seafood.

AB - This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid group ranged from 69% (raw European razor clams) to 74% (raw donax clams). Regarding azaspiracids, 47% of the initial content was bioaccessible in steamed blue mussel. As for hydrophilic toxins, 100% of the initial content was bioaccessible after digestion in raw shellfish and puffer fish gonads. The total tetrodotoxin bioaccessibility in puffer fish gonads decreased significantly after steaming. The profile of tetrodotoxins changed during the digestion process: TTX and 11-norTTX-6S-ol analogues decreased significantly after digestion, but the 5,6,11-trideoxy TTX analogue increased in both raw and steamed puffer fish gonads. These preliminary findings confirm the need to consider bioaccessibility data in future seafood risk assessment, as such information enables a more accurate and realistic estimation of potential seafood hazards, particularly in what concerns lipophilic toxins, therefore, constituting a crucial tool in the refinement of regulatory limits for the presence of biotoxins in seafood.

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DO - 10.1016/j.fct.2019.04.041

M3 - Article

SN - 1873-6351

VL - 129

SP - 153

EP - 161

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

ER -

Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach

Abstract

Bibliographical note

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