Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction

Sakandar Rauf; Veerappan Mani; Abdellatif Ait Lahcen; Saravanan Yuvaraja; Tutku Beduk; Khaled N. Salama

doi:10.1016/j.electacta.2021.138489

Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction

Sakandar Rauf, Veerappan Mani, Abdellatif Ait Lahcen, Saravanan Yuvaraja, Tutku Beduk, Khaled N. Salama

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.

Original language	English (US)
Pages (from-to)	138489
Journal	Electrochimica Acta
Volume	386
DOIs	https://doi.org/10.1016/j.electacta.2021.138489
State	Published - May 1 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-05-27
Acknowledged KAUST grant number(s): KAUST Sensor Initiative
Acknowledgements: The authors would like to express their acknowledgments to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative for supporting this work. In addition, we would like to thank Dr. Govindasamy Mani for his valuable suggestions regarding material synthesis.

ASJC Scopus subject areas

General Chemical Engineering
Electrochemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.electacta.2021.138489

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title = "Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction",

abstract = "Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.",

author = "Sakandar Rauf and Veerappan Mani and {Ait Lahcen}, Abdellatif and Saravanan Yuvaraja and Tutku Beduk and Salama, {Khaled N.}",

note = "KAUST Repository Item: Exported on 2021-05-27 Acknowledged KAUST grant number(s): KAUST Sensor Initiative Acknowledgements: The authors would like to express their acknowledgments to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative for supporting this work. In addition, we would like to thank Dr. Govindasamy Mani for his valuable suggestions regarding material synthesis.",

year = "2021",

month = may,

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doi = "10.1016/j.electacta.2021.138489",

language = "English (US)",

volume = "386",

pages = "138489",

journal = "Electrochimica Acta",

issn = "0013-4686",

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TY - JOUR

T1 - Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction

AU - Rauf, Sakandar

AU - Mani, Veerappan

AU - Ait Lahcen, Abdellatif

AU - Yuvaraja, Saravanan

AU - Beduk, Tutku

AU - Salama, Khaled N.

N1 - KAUST Repository Item: Exported on 2021-05-27 Acknowledged KAUST grant number(s): KAUST Sensor Initiative Acknowledgements: The authors would like to express their acknowledgments to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative for supporting this work. In addition, we would like to thank Dr. Govindasamy Mani for his valuable suggestions regarding material synthesis.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.

AB - Laser-scribed graphene (LSG) electrodes have gained significant interest due to the ease in fabrication, surface modification, and potential to develop various types of electrochemical sensors and biosensors. In these studies, a new type of zinc ferrite nanoparticles (ZnFe2O4 NPs) modified LSG electrochemical sensing system comprising LSG-ZnFe2O4 working electrode, LSG reference, and LSG counter electrode on a single polyimide substrate is presented. LSG-ZnFe2O4 electrodes are fabricated by drop-casting a solution of ZnFe2O4 NPs onto the LSG electrode, which gave a 31% enhancement of sensitivity and electrocatalytic activity compared to the bare LSG electrode. LSG-ZnFe2O4 electrochemical aptasensor for acute myocardial infarction (AMI) screening is developed by detecting the cardiac Troponin-I (cTn-I) biomarker. The results show that the developed aptasensor could detect a broad concentration range of cTn-I with a limit of detection of 0.001 ng/mL and a sensitivity of 19.32 (±0.25) µA/(ng/mL). In addition to this, LSG-ZnFe2O4-aptasensor shows higher selectivity towards the detection of cTn-I and negligible cross-reactivity with other interfering biomolecules. Finally, it is demonstrated that LSG-ZnFe2O4-aptasensor can easily detect different concentrations of cTn-I spiked in human serum samples. These results show that the LSG-ZnFe2O4-aptasensor is a promising diagnostic tool to monitor cTn-I and could be a potential candidate to develop point-of-care devices for cTn-I biomarker detection and various other disease biomarkers in the future.

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UR - https://linkinghub.elsevier.com/retrieve/pii/S0013468621007799

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U2 - 10.1016/j.electacta.2021.138489

DO - 10.1016/j.electacta.2021.138489

M3 - Article

SN - 0013-4686

VL - 386

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JO - Electrochimica Acta

JF - Electrochimica Acta

ER -

Binary transition metal oxide modified laser-scribed graphene electrochemical aptasensor for the accurate and sensitive screening of acute myocardial infarction

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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