Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil

João Ricardo Vidal Amaral; Rommel Thiago Jucá Ramos; Fabrício Almeida Araújo; Rodrigo Bentes Kato; Flávia Figueira Aburjaile; Siomar de Castro Soares; Aristóteles Góes-Neto; Mateus Matiuzzi da Costa; Vasco Azevedo; Bertram Brenig; Selma Soares de Oliveira; Alexandre S. Rosado

doi:10.3390/microorganisms10030588

Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil

João Ricardo Vidal Amaral, Rommel Thiago Jucá Ramos, Fabrício Almeida Araújo, Rodrigo Bentes Kato, Flávia Figueira Aburjaile, Siomar de Castro Soares, Aristóteles Góes-Neto, Mateus Matiuzzi da Costa, Vasco Azevedo, Bertram Brenig, Selma Soares de Oliveira, Alexandre S. Rosado

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus—namely, S. pyogenes, S. agalactiae, S. porcinus, and S. uberis. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.

Original language	English (US)
Pages (from-to)	588
Journal	Microorganisms
Volume	10
Issue number	3
DOIs	https://doi.org/10.3390/microorganisms10030588
State	Published - Mar 9 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-04-26
Acknowledged KAUST grant number(s): BAS/1/1096-01-01
Acknowledgements: We would like to acknowledge the Brazilian funding agency Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the scholarship granted. We would also like to thank Maria Aparecida Vasconcelos Paiva Brito from the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) for supplying the Streptococcus agalactiae strains studied in this research. Furthermore, we thank Maria do Carmo de Freire Bastos, Marco Antônio Lemos Miguel, and Lúcia Martins Teixeira from the Universidade Federal do Rio de Janeiro for providing indicator bacteria strains for our study. : This research was funded by “Rede de Ciências Ômicas”—RECOM (Network of Omics’
Sciences) and KAUST grant number (BAS/1/1096-01-01) and the APC was funded by KAUST.

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https://repository.kaust.edu.sa/bitstream/10754/676478/1/microorganisms-10-00588.pdf

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Vidal Amaral, J. R., Jucá Ramos, R. T., Almeida Araújo, F., Bentes Kato, R., Figueira Aburjaile, F., de Castro Soares, S., Góes-Neto, A., Matiuzzi da Costa, M., Azevedo, V., Brenig, B., Oliveira, S. S. D., & Rosado, A. S. (2022). Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil. Microorganisms, 10(3), 588. https://doi.org/10.3390/microorganisms10030588

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title = "Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil",

abstract = "Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus—namely, S. pyogenes, S. agalactiae, S. porcinus, and S. uberis. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.",

author = "{Vidal Amaral}, {Jo{\~a}o Ricardo} and {Juc{\'a} Ramos}, {Rommel Thiago} and {Almeida Ara{\'u}jo}, Fabr{\'i}cio and {Bentes Kato}, Rodrigo and {Figueira Aburjaile}, Fl{\'a}via and {de Castro Soares}, Siomar and Arist{\'o}teles G{\'o}es-Neto and {Matiuzzi da Costa}, Mateus and Vasco Azevedo and Bertram Brenig and Oliveira, {Selma Soares de} and Rosado, {Alexandre S.}",

note = "KAUST Repository Item: Exported on 2022-04-26 Acknowledged KAUST grant number(s): BAS/1/1096-01-01 Acknowledgements: We would like to acknowledge the Brazilian funding agency Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) for the scholarship granted. We would also like to thank Maria Aparecida Vasconcelos Paiva Brito from the Empresa Brasileira de Pesquisa Agropecu{\'a}ria (Embrapa) for supplying the Streptococcus agalactiae strains studied in this research. Furthermore, we thank Maria do Carmo de Freire Bastos, Marco Ant{\^o}nio Lemos Miguel, and L{\'u}cia Martins Teixeira from the Universidade Federal do Rio de Janeiro for providing indicator bacteria strains for our study. : This research was funded by “Rede de Ci{\^e}ncias {\^O}micas”—RECOM (Network of Omics{\textquoteright} Sciences) and KAUST grant number (BAS/1/1096-01-01) and the APC was funded by KAUST.",

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doi = "10.3390/microorganisms10030588",

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Vidal Amaral, JR, Jucá Ramos, RT, Almeida Araújo, F, Bentes Kato, R, Figueira Aburjaile, F, de Castro Soares, S, Góes-Neto, A, Matiuzzi da Costa, M, Azevedo, V, Brenig, B, Oliveira, SSD & Rosado, AS 2022, 'Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil', Microorganisms, vol. 10, no. 3, pp. 588. https://doi.org/10.3390/microorganisms10030588

TY - JOUR

T1 - Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil

AU - Vidal Amaral, João Ricardo

AU - Jucá Ramos, Rommel Thiago

AU - Almeida Araújo, Fabrício

AU - Bentes Kato, Rodrigo

AU - Figueira Aburjaile, Flávia

AU - de Castro Soares, Siomar

AU - Góes-Neto, Aristóteles

AU - Matiuzzi da Costa, Mateus

AU - Azevedo, Vasco

AU - Brenig, Bertram

AU - Oliveira, Selma Soares de

AU - Rosado, Alexandre S.

N1 - KAUST Repository Item: Exported on 2022-04-26 Acknowledged KAUST grant number(s): BAS/1/1096-01-01 Acknowledgements: We would like to acknowledge the Brazilian funding agency Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the scholarship granted. We would also like to thank Maria Aparecida Vasconcelos Paiva Brito from the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) for supplying the Streptococcus agalactiae strains studied in this research. Furthermore, we thank Maria do Carmo de Freire Bastos, Marco Antônio Lemos Miguel, and Lúcia Martins Teixeira from the Universidade Federal do Rio de Janeiro for providing indicator bacteria strains for our study. : This research was funded by “Rede de Ciências Ômicas”—RECOM (Network of Omics’ Sciences) and KAUST grant number (BAS/1/1096-01-01) and the APC was funded by KAUST.

PY - 2022/3/9

Y1 - 2022/3/9

N2 - Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus—namely, S. pyogenes, S. agalactiae, S. porcinus, and S. uberis. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.

AB - Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus—namely, S. pyogenes, S. agalactiae, S. porcinus, and S. uberis. Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.

UR - http://hdl.handle.net/10754/676478

UR - https://www.mdpi.com/2076-2607/10/3/588

UR - http://www.scopus.com/inward/record.url?scp=85126030722&partnerID=8YFLogxK

U2 - 10.3390/microorganisms10030588

DO - 10.3390/microorganisms10030588

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SN - 2076-2607

VL - 10

SP - 588

JO - Microorganisms

JF - Microorganisms

IS - 3

ER -

Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil

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