Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

Melina Zourelidou; Birgit Absmanner; Benjamin Weller; Inês CR Barbosa; Björn C Willige; Astrid Fastner; Verena Streit; Sarah A Port; Jean Colcombet; Sergio de la Fuente van Bentem; Heribert Hirt; Bernhard Kuster; Waltraud X Schulze; Ulrich Z Hammes; Claus Schwechheimer

doi:10.7554/eLife.02860

Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

Melina Zourelidou, Birgit Absmanner, Benjamin Weller, Inês CR Barbosa, Björn C Willige, Astrid Fastner, Verena Streit, Sarah A Port, Jean Colcombet, Sergio de la Fuente van Bentem, Heribert Hirt, Bernhard Kuster, Waltraud X Schulze, Ulrich Z Hammes, Claus Schwechheimer

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206 Scopus citations

Abstract

The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

Original language	English (US)
Journal	eLife
Volume	3
Issue number	3
DOIs	https://doi.org/10.7554/eLife.02860
State	Published - Jun 19 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Neuroscience

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10.7554/eLife.02860

https://repository.kaust.edu.sa/bitstream/10754/334618/1/Article-eLife-Auxin_effl-2014.pdf

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Zourelidou, M., Absmanner, B., Weller, B., Barbosa, I. CR., Willige, B. C., Fastner, A., Streit, V., Port, S. A., Colcombet, J., de la Fuente van Bentem, S., Hirt, H., Kuster, B., Schulze, W. X., Hammes, U. Z., & Schwechheimer, C. (2014). Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. eLife, 3(3). https://doi.org/10.7554/eLife.02860

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title = "Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID",

abstract = "The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.",

author = "Melina Zourelidou and Birgit Absmanner and Benjamin Weller and Barbosa, {In{\^e}s CR} and Willige, {Bj{\"o}rn C} and Astrid Fastner and Verena Streit and Port, {Sarah A} and Jean Colcombet and {de la Fuente van Bentem}, Sergio and Heribert Hirt and Bernhard Kuster and Schulze, {Waltraud X} and Hammes, {Ulrich Z} and Claus Schwechheimer",

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Zourelidou, M, Absmanner, B, Weller, B, Barbosa, ICR, Willige, BC, Fastner, A, Streit, V, Port, SA, Colcombet, J, de la Fuente van Bentem, S, Hirt, H, Kuster, B, Schulze, WX, Hammes, UZ & Schwechheimer, C 2014, 'Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID', eLife, vol. 3, no. 3. https://doi.org/10.7554/eLife.02860

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AU - Zourelidou, Melina

AU - Absmanner, Birgit

AU - Weller, Benjamin

AU - Barbosa, Inês CR

AU - Willige, Björn C

AU - Fastner, Astrid

AU - Streit, Verena

AU - Port, Sarah A

AU - Colcombet, Jean

AU - de la Fuente van Bentem, Sergio

AU - Hirt, Heribert

AU - Kuster, Bernhard

AU - Schulze, Waltraud X

AU - Hammes, Ulrich Z

AU - Schwechheimer, Claus

N1 - KAUST Repository Item: Exported on 2020-10-01

PY - 2014/6/19

Y1 - 2014/6/19

N2 - The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

AB - The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

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SN - 2050-084X

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JO - eLife

JF - eLife

IS - 3

ER -

Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

Abstract

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