Attempting to synthesize lasso peptides using high pressure

Mateusz Waliczek; Magdalena Wierzbicka; Maciej Arkuszewski; Monika Kijewska; Lukasz Jaremko; Priyadharshni Rajagopal; Kacper Szczepski; Amanda Sroczyńska; Mariusz Jaremko; Piotr Stefanowicz

doi:10.1371/journal.pone.0234901

Attempting to synthesize lasso peptides using high pressure

Mateusz Waliczek, Magdalena Wierzbicka, Maciej Arkuszewski, Monika Kijewska, Lukasz Jaremko, Priyadharshni Rajagopal, Kacper Szczepski, Amanda Sroczyńska, Mariusz Jaremko, Piotr Stefanowicz

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14000 kbar.

Original language	English (US)
Pages (from-to)	e0234901
Journal	PLOS ONE
Volume	15
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0234901
State	Published - Jun 24 2020

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by Grant No.UMO-2015/19/B/ST5/00659 from the National Science Centre, Poland https://www.ncn.gov.pl/ The co-authors: Łukasz Jaremko, Mariusz Jaremko, Priyadharshni Rajagopal and Kacper Szczepski are grateful to King Abdullah University of Science and Technology (KAUST) for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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10.1371/journal.pone.0234901

https://repository.kaust.edu.sa/bitstream/10754/663847/1/journal.pone.0234901.pdf

Cite this

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title = "Attempting to synthesize lasso peptides using high pressure",

abstract = "Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14000 kbar.",

author = "Mateusz Waliczek and Magdalena Wierzbicka and Maciej Arkuszewski and Monika Kijewska and Lukasz Jaremko and Priyadharshni Rajagopal and Kacper Szczepski and Amanda Sroczy{\'n}ska and Mariusz Jaremko and Piotr Stefanowicz",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by Grant No.UMO-2015/19/B/ST5/00659 from the National Science Centre, Poland https://www.ncn.gov.pl/ The co-authors: {\L}ukasz Jaremko, Mariusz Jaremko, Priyadharshni Rajagopal and Kacper Szczepski are grateful to King Abdullah University of Science and Technology (KAUST) for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.",

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doi = "10.1371/journal.pone.0234901",

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AU - Waliczek, Mateusz

AU - Wierzbicka, Magdalena

AU - Arkuszewski, Maciej

AU - Kijewska, Monika

AU - Jaremko, Lukasz

AU - Rajagopal, Priyadharshni

AU - Szczepski, Kacper

AU - Sroczyńska, Amanda

AU - Jaremko, Mariusz

AU - Stefanowicz, Piotr

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by Grant No.UMO-2015/19/B/ST5/00659 from the National Science Centre, Poland https://www.ncn.gov.pl/ The co-authors: Łukasz Jaremko, Mariusz Jaremko, Priyadharshni Rajagopal and Kacper Szczepski are grateful to King Abdullah University of Science and Technology (KAUST) for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

PY - 2020/6/24

Y1 - 2020/6/24

N2 - Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14000 kbar.

AB - Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14000 kbar.

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JO - PLOS ONE

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ER -

Attempting to synthesize lasso peptides using high pressure

Abstract

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