AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation

Monique Morse; Ganka Pironcheva; Chris Gehring

doi:10.1016/S0014-5793(03)01384-X

AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation

Monique Morse, Ganka Pironcheva, Chris Gehring^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full-length recombinant (AtPNP-A[1-126]) and demonstrate that this molecule induces osmoticum-dependent H₂O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP-A[26-126]) as well as a short domain (AtPNP-A[33-66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP-A has an important and systemic role in plant growth and homeostasis.

Original language	English (US)
Pages (from-to)	99-103
Number of pages	5
Journal	FEBS Letters
Volume	556
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)01384-X
State	Published - Jan 2 2004
Externally published	Yes

Bibliographical note

Funding Information:
This project was supported by a South African National Research Foundation (NRF) grant to C.G. and M.M. receives a NRF doctoral bursary.

Keywords

Arabidopsis thaliana
Cell volume regulation
Natriuretic peptide
Plant homeostasis
Plant protoplast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(03)01384-X

Cite this

@article{fc4e74fc0553411dbdbe1d4dee728012,

title = "AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation",

abstract = "Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full-length recombinant (AtPNP-A[1-126]) and demonstrate that this molecule induces osmoticum-dependent H2O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP-A[26-126]) as well as a short domain (AtPNP-A[33-66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP-A has an important and systemic role in plant growth and homeostasis.",

keywords = "Arabidopsis thaliana, Cell volume regulation, Natriuretic peptide, Plant homeostasis, Plant protoplast",

author = "Monique Morse and Ganka Pironcheva and Chris Gehring",

note = "Funding Information: This project was supported by a South African National Research Foundation (NRF) grant to C.G. and M.M. receives a NRF doctoral bursary.",

year = "2004",

month = jan,

day = "2",

doi = "10.1016/S0014-5793(03)01384-X",

language = "English (US)",

volume = "556",

pages = "99--103",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1-3",

}

TY - JOUR

T1 - AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation

AU - Morse, Monique

AU - Pironcheva, Ganka

AU - Gehring, Chris

N1 - Funding Information: This project was supported by a South African National Research Foundation (NRF) grant to C.G. and M.M. receives a NRF doctoral bursary.

PY - 2004/1/2

Y1 - 2004/1/2

N2 - Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full-length recombinant (AtPNP-A[1-126]) and demonstrate that this molecule induces osmoticum-dependent H2O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP-A[26-126]) as well as a short domain (AtPNP-A[33-66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP-A has an important and systemic role in plant growth and homeostasis.

AB - Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full-length recombinant (AtPNP-A[1-126]) and demonstrate that this molecule induces osmoticum-dependent H2O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP-A[26-126]) as well as a short domain (AtPNP-A[33-66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP-A has an important and systemic role in plant growth and homeostasis.

KW - Arabidopsis thaliana

KW - Cell volume regulation

KW - Natriuretic peptide

KW - Plant homeostasis

KW - Plant protoplast

UR - http://www.scopus.com/inward/record.url?scp=0346094391&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(03)01384-X

DO - 10.1016/S0014-5793(03)01384-X

M3 - Article

C2 - 14706834

AN - SCOPUS:0346094391

SN - 0014-5793

VL - 556

SP - 99

EP - 103

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this