Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans

Karen L Artiles; Andrew Z Fire; Christian Frøkjær-Jensen

doi:10.1016/j.devcel.2019.01.020

Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans

Karen L Artiles, Andrew Z Fire, Christian Frøkjær-Jensen

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10 Scopus citations

Abstract

The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.

Original language	English (US)
Pages (from-to)	827-839.e9
Journal	Developmental Cell
Volume	48
Issue number	6
DOIs	https://doi.org/10.1016/j.devcel.2019.01.020
State	Published - Feb 23 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1(OE) strains and their genetic interactions; Josh Arribere for the gfp-tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS-R01-GM37706/GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440).

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10.1016/j.devcel.2019.01.020

https://repository.kaust.edu.sa/bitstream/10754/631308/1/Artilles%20et%20al%202019%20Dev%20Cell%20R1%20accepted%20version.pdf

Cite this

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title = "Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans",

abstract = "The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.",

author = "Artiles, {Karen L} and Fire, {Andrew Z} and Christian Fr{\o}kj{\ae}r-Jensen",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1(OE) strains and their genetic interactions; Josh Arribere for the gfp-tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS-R01-GM37706/GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440).",

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N2 - The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.

AB - The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.

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JO - Developmental Cell

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ER -

Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans

Abstract

Bibliographical note

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