Coral reefs are declining due to anthropogenic disturbances, including climate change. Therefore, improving our understanding of coral ecosystems is vital, and the influence of bacteria on coral health has attracted particular interest. However, a gnotobiotic coral model that could enhance studies of coral–bacteria interactions is absent. To address this gap, we tested the ability of treatment with seven antibiotics for 3 weeks to deplete bacteria in Exaiptasia diaphana, a sea anemone widely used as a coral model. Digital droplet PCR (ddPCR) targeting anemone Ef1-α and bacterial 16S rRNA genes was used to quantify bacterial load, which was found to decrease six-fold. However, metabarcoding of bacterial 16S rRNA genes showed that alpha and beta diversity of the anemone-associated bacterial communities increased significantly. Therefore, gnotobiotic E. diaphana with simplified, uniform bacterial communities were not generated, with biofilm formation in the culture vessels most likely impeding efforts to eliminate bacteria. Despite this outcome, our work will inform future efforts to create a much needed gnotobiotic coral model.
Bibliographical noteKAUST Repository Item: Exported on 2022-01-26
Acknowledgements: The authors thank Dr Ruben Costa and Prof. Christian Voolstra from the King Abdullah University of Science and Technology (KAUST) for sharing a draft version of their gnotobiotic Aiptasia protocol. L.M.H. also thanks Ms Franca Casagranda from the University of Melbourne for her assistance in developing the ddPCR B/H assay.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.