TY - JOUR
T1 - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
AU - Vercruyssen, Liesbeth
AU - Verkest, Aurine
AU - Gonzalez, Nathalie
AU - Heyndrickx, Ken S.
AU - Eeckhout, Dominique
AU - Han, Soon Ki
AU - Jégu, Teddy
AU - Archacki, Rafal
AU - Van Leene, Jelle
AU - Andriankaja, Megan
AU - De Bodt, Stefanie
AU - Abeel, Thomas
AU - Coppens, Frederik
AU - Dhondt, Stijn
AU - De Milde, Liesbeth
AU - Vermeersch, Mattias
AU - Maleux, Katrien
AU - Gevaert, Kris
AU - Jerzmanowski, Andrzej
AU - Benhamed, Moussa
AU - Wagner, Doris
AU - Vandepoele, Klaas
AU - De Jaeger, Geert
AU - Inzé, Dirk
PY - 2014/1
Y1 - 2014/1
N2 - The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.
AB - The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.
UR - http://www.scopus.com/inward/record.url?scp=84896899026&partnerID=8YFLogxK
U2 - 10.1105/tpc.113.115907
DO - 10.1105/tpc.113.115907
M3 - Article
C2 - 24443518
AN - SCOPUS:84896899026
SN - 1040-4651
VL - 26
SP - 210
EP - 229
JO - Plant Cell
JF - Plant Cell
IS - 1
ER -