Analyzing Lysosome-Related Organelles by Electron Microscopy

Ilse Hurbain; Maryse Romao; Ptissam Bergam; Xavier Heiligenstein; Graça Raposo

doi:10.1007/978-1-4939-6934-0_4

Analyzing Lysosome-Related Organelles by Electron Microscopy

Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, Graça Raposo

Research output: Chapter in Book/Report/Conference proceeding › Chapter

24 Scopus citations

Abstract

Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Springer Nature
Pages	43-71
Number of pages	29
ISBN (Print)	9781493969326
DOIs	https://doi.org/10.1007/978-1-4939-6934-0_4
State	Published - Apr 30 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.

ASJC Scopus subject areas

Genetics
Molecular Biology

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10.1007/978-1-4939-6934-0_4

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abstract = "Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.",

author = "Ilse Hurbain and Maryse Romao and Ptissam Bergam and Xavier Heiligenstein and Gra{\c c}a Raposo",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche M{\'e}dical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L{\textquoteright}Or{\'e}al.",

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AU - Hurbain, Ilse

AU - Romao, Maryse

AU - Bergam, Ptissam

AU - Heiligenstein, Xavier

AU - Raposo, Graça

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.

PY - 2017/4/30

Y1 - 2017/4/30

N2 - Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.

AB - Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.

UR - http://hdl.handle.net/10754/623792

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M3 - Chapter

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SP - 43

EP - 71

BT - Methods in Molecular Biology

PB - Springer Nature

ER -

Analyzing Lysosome-Related Organelles by Electron Microscopy

Abstract

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