Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Dec 5 2002|
Bibliographical noteFunding Information:
Acknowledgements We thank the following (listed in alphabetical order) for discussion, encouragement and technical assistance: K. Abe, T. Akimura, T. Hanagaki, K. Hayashida, K. Hiramoto, T. Hiraoka, M. Honda, F. Hori, T. Huber, M. Izawa, H. Kato, M. Kohda, Y. Kojima, S. Koya, K. Koyama, C. Kurihara, J. Mashima, T. Matsuyama, M. Murata, K. Nishi, K. Nomura, R. Numazaki, M. Ohno, H. Saitou, C. Sakai, H. Sano, Y. Shibata, A. J. G. Simpson, Y. Sogabe, M. Tagami, A. Tagawa, F. Takahashi, S. Takaku, Y. Takeda, T. Tanaka, A. Tomaru, W. W. Wasserman, A. Watahiki, C. Wicking, H. Yamanishi, T. Yao, K. Yoshida. We especially thank A. Wada, M. Muramatsu, T. Ogawa, A. Kira, and all the members of RIKEN Yokohama Research Promotion Division for supporting and encouraging the project. We also thank the Laboratory for Genome Exploration Research Group for secretarial and technical assistance. This work was mainly supported by a research grant for the RIKEN Genome Exploration Research Project from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of the Japanese Government to Y.H., and by a Research and Development for Applying Advanced Computational Science and Technology (ACT) grant from the Japan Science and Technology Corporation to Y.H. and J.K.
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