Analysis of Pacific oyster larval proteome and its response to high-CO2

R. Dineshram; Kelvin K.W. Wong; Shu Xiao; Ziniu Yu; Pei-Yuan Qian; Vengatesen Thiyagarajan

doi:10.1016/j.marpolbul.2012.07.043

Analysis of Pacific oyster larval proteome and its response to high-CO2

R. Dineshram, Kelvin K.W. Wong, Shu Xiao, Ziniu Yu, Pei-Yuan Qian, Vengatesen Thiyagarajan

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. © 2012 Elsevier Ltd.

Original language	English (US)
Pages (from-to)	2160-2167
Number of pages	8
Journal	Marine Pollution Bulletin
Volume	64
Issue number	10
DOIs	https://doi.org/10.1016/j.marpolbul.2012.07.043
State	Published - Oct 2012
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We thank A. Ishimatsu (Nagasaki University, Japan), J.M. Hall-Spencer (University of Plymouth, UK), Sam Dupont (EPOCA, Sweden), Richard Zeebe (University of Hawaii, USA), Gray Williams and Kenneth Leung (The University of Hong Kong, Hong Kong) for their valuable discussions and for their support in setting up the ocean acidification facilities. We thank Mr. Fu (in oyster hatchery) for his support on oyster larval culture. This study was primarily supported by a Grant from the HKSAR-RGC (No. 778309M) and partially from the Area of Excellency Project grant (No. AoE/P-04/2004) to VT. The oyster larval culture facilities located in Zhanjiang (China) hatchery was partially funded by the 973 program to ZY. The proteomic part of this study was partially supported by KAUST-HKUST project funded to PYQ.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.marpolbul.2012.07.043

Cite this

@article{3d92f25537164e089346ae8d1e18fb9b,

title = "Analysis of Pacific oyster larval proteome and its response to high-CO2",

abstract = "Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. {\textcopyright} 2012 Elsevier Ltd.",

author = "R. Dineshram and Wong, {Kelvin K.W.} and Shu Xiao and Ziniu Yu and Pei-Yuan Qian and Vengatesen Thiyagarajan",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We thank A. Ishimatsu (Nagasaki University, Japan), J.M. Hall-Spencer (University of Plymouth, UK), Sam Dupont (EPOCA, Sweden), Richard Zeebe (University of Hawaii, USA), Gray Williams and Kenneth Leung (The University of Hong Kong, Hong Kong) for their valuable discussions and for their support in setting up the ocean acidification facilities. We thank Mr. Fu (in oyster hatchery) for his support on oyster larval culture. This study was primarily supported by a Grant from the HKSAR-RGC (No. 778309M) and partially from the Area of Excellency Project grant (No. AoE/P-04/2004) to VT. The oyster larval culture facilities located in Zhanjiang (China) hatchery was partially funded by the 973 program to ZY. The proteomic part of this study was partially supported by KAUST-HKUST project funded to PYQ. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2012",

month = oct,

doi = "10.1016/j.marpolbul.2012.07.043",

language = "English (US)",

volume = "64",

pages = "2160--2167",

journal = "Marine Pollution Bulletin",

issn = "0025-326X",

publisher = "Elsevier",

number = "10",

}

TY - JOUR

T1 - Analysis of Pacific oyster larval proteome and its response to high-CO2

AU - Dineshram, R.

AU - Wong, Kelvin K.W.

AU - Xiao, Shu

AU - Yu, Ziniu

AU - Qian, Pei-Yuan

AU - Thiyagarajan, Vengatesen

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: We thank A. Ishimatsu (Nagasaki University, Japan), J.M. Hall-Spencer (University of Plymouth, UK), Sam Dupont (EPOCA, Sweden), Richard Zeebe (University of Hawaii, USA), Gray Williams and Kenneth Leung (The University of Hong Kong, Hong Kong) for their valuable discussions and for their support in setting up the ocean acidification facilities. We thank Mr. Fu (in oyster hatchery) for his support on oyster larval culture. This study was primarily supported by a Grant from the HKSAR-RGC (No. 778309M) and partially from the Area of Excellency Project grant (No. AoE/P-04/2004) to VT. The oyster larval culture facilities located in Zhanjiang (China) hatchery was partially funded by the 973 program to ZY. The proteomic part of this study was partially supported by KAUST-HKUST project funded to PYQ. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2012/10

Y1 - 2012/10

N2 - Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. © 2012 Elsevier Ltd.

AB - Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. © 2012 Elsevier Ltd.

UR - http://hdl.handle.net/10754/597560

UR - https://linkinghub.elsevier.com/retrieve/pii/S0025326X12003645

UR - http://www.scopus.com/inward/record.url?scp=84866772895&partnerID=8YFLogxK

U2 - 10.1016/j.marpolbul.2012.07.043

DO - 10.1016/j.marpolbul.2012.07.043

M3 - Article

C2 - 22921897

SN - 0025-326X

VL - 64

SP - 2160

EP - 2167

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 10

ER -

Analysis of Pacific oyster larval proteome and its response to high-CO2

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this