Analysis of different coating thickness on new type of planar interdigital sensors for endotoxin detection

A. R Mohd Syaifudin, Paklam Yu, Subhas Chandra Mukhopadhyay, Chinthaka Pasan Gooneratne, Jürgen Kosel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

New types of planar interdigital sensors have been fabricated on Silicon/Silicon Dioxide (Si/SiO2) wafers. The sensors were coated with pre-cursor silica functionalized with APTES (3-aminopropyltrietoxysilane) at different thicknesses. All sensors were then immobilized with Polymyxin, B (PmB). PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus, has been immobilized on the coated sensors because of its specific binding properties to endotoxin. Studies were conducted to analyze the effect of different thicknesses of coatings on the sensitivity and selectivity of the sensors. It was observed sensors coated with 3 layers of coating has better sensitivity and selectivity to the target molecules (endotoxin) compared to sensors with 5 layers of coating. The repeatability and stability of the coated sensors were tested by multiple standard endotoxin measurement and it was observed that the sensors give a good reproducibility and stability up to six continuous measurements before the coating degrades. © 2013 IEEE.
Original languageEnglish (US)
Title of host publication2013 IEEE International Instrumentation and Measurement Technology Conference (I2MTC)
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages1496-1501
Number of pages6
ISBN (Print)9781467346221
DOIs
StatePublished - May 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01

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