Analysis of dicarboxylic acids by tandem mass spectrometry. High-throughput quantitative measurement of methylmalonic acid in serum, plasma, and urine

M. M. Kushnir, G. Komaromy-Hiller, B. Shushan, F. M. Urry, W. L. Roberts

Research output: Contribution to journalArticlepeer-review

142 Scopus citations

Abstract

Background: Methylmalonic acid (MMA) is a dicarboxylic acid whose concentration can be increased in blood and urine in patients with an inborn error of metabolism or vitamin B12 deficiency. We developed a method for the selective analysis of dicarboxylic acids that exploits the high specificity of tandem mass spectrometry (MS/MS) and the substantial difference in fragmentation patterns of the isomers methylmalonic (MMA) and succinic acid (SA). Methods: Dicarboxylic acids were extracted from samples with methyl-tert-butyl ether and derivatized with butanolic HCl to form dibutyl esters. The derivative was injected into the liquid chromatography (LC)-MS/MS system using TurboIonSpray™ (nebulizer-assisted electrospray) ionization and quantified by the multiple reaction monitoring mode of MS/MS. Results: The assay for MMA was linear up to 150 μmol/L. The total imprecision was ≤7.5% at both low and high concentrations. The limits of quantification and detection were 0.1 and 0.05 μmol/L, respectively. The degree of interference from SA could be predicted from the branching ratios of the major product ions. Conclusions: The method is specific for dicarboxylic acids. The LC-MS/MS analysis for MMA requires minimal chromatographic separation and takes
Original languageEnglish (US)
Pages (from-to)1993-2002
Number of pages10
JournalClinical Chemistry
Volume47
Issue number11
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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