Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet; B. Rappaz; F. Charrière; Y. Emery; C. Depeursinge; P. Magistretti

Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet, B. Rappaz, F. Charrière, Y. Emery, C. Depeursinge, P. Magistretti

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

Original language	English (US)
Title of host publication	European Conference on Biomedical Optics, ECBO 2007
Publisher	Optical Society of America
ISBN (Print)	9780819467713
State	Published - 2007
Externally published	Yes
Event	European Conference on Biomedical Optics, ECBO 2007 - Munich, Germany Duration: Jun 17 2007 → Jun 17 2007

Other

Other	European Conference on Biomedical Optics, ECBO 2007
Country/Territory	Germany
City	Munich
Period	06/17/07 → 06/17/07

Keywords

Cell imaging
Cell morphology
Digital holography
Refractive index

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

Cite this

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title = "Analysis of cellular structure and dynamics with digital holographic microscopy",

abstract = "Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.",

keywords = "Cell imaging, Cell morphology, Digital holography, Refractive index",

author = "P. Marquet and B. Rappaz and F. Charri{\`e}re and Y. Emery and C. Depeursinge and P. Magistretti",

year = "2007",

language = "English (US)",

isbn = "9780819467713",

booktitle = "European Conference on Biomedical Optics, ECBO 2007",

publisher = "Optical Society of America",

note = "European Conference on Biomedical Optics, ECBO 2007 ; Conference date: 17-06-2007 Through 17-06-2007",

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TY - GEN

T1 - Analysis of cellular structure and dynamics with digital holographic microscopy

AU - Marquet, P.

AU - Rappaz, B.

AU - Charrière, F.

AU - Emery, Y.

AU - Depeursinge, C.

AU - Magistretti, P.

PY - 2007

Y1 - 2007

N2 - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

AB - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

KW - Cell imaging

KW - Cell morphology

KW - Digital holography

KW - Refractive index

UR - http://www.scopus.com/inward/record.url?scp=84898770843&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84898770843

SN - 9780819467713

BT - European Conference on Biomedical Optics, ECBO 2007

PB - Optical Society of America

T2 - European Conference on Biomedical Optics, ECBO 2007

Y2 - 17 June 2007 through 17 June 2007

ER -

Analysis of cellular structure and dynamics with digital holographic microscopy

Abstract

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Keywords

ASJC Scopus subject areas

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