An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal Hoe Koo, Miroslava Karafiátová, István Molnár, Kateřina Holušová, Jaroslav Doležel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa, Łukasz Jaremko, Jesse Poland, Simon G. Krattinger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with disease-resistance genes 1. Here we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata 2. We established that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Aegilops triuncialis 3, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.

Original languageEnglish (US)
Pages (from-to)914-920
Number of pages7
JournalNature Genetics
Volume55
Issue number6
DOIs
StatePublished - Jun 2023

Bibliographical note

Funding Information:
We are grateful to L. Zou (KAUST) for technical assistance, Y. Zhou (KAUST) and H.I. Ahmed (KAUST) for discussions on bioinformatics analyses and the KAUST Bioscience Core Lab for sequencing support. We thank Z. Dubská, R. Šperková and J. Weiserová (Institute of Experimental Botany) for the preparation of chromosome samples for flow cytometry, M. Said and P. Cápál (Institute of Experimental Botany) for chromosome 2B sorting and J. Raupp from the Wheat Genetics Resource Center for providing germplasm. We thank J. A. Kolmer from the USDA-ARS Cereal Disease Laboratory, St. Paul, MN, for providing P. triticina isolate MNPSD. We thank P. Lu from IGDB, CAS, for providing TKP sequences for phylogenetic analysis. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award OSR-CRG2018-3768. D.K. was supported by WGRC/IUCRC and NSF (grant 1822162). I.M. received support from the Marie Curie Fellowship grant ‘AEGILWHEAT’ (H2020-MSCA-IF-2016-746253) and from the Hungarian National Research, Development and Innovation Office (K135057). M.K., K.H. and J.D. received support from the ERDF project 'Plants as a tool for sustainable global development' (CZ.02.1.01/0.0/0.0/16_019/0000827). Computational resources were supplied by the project 'e-Infrastruktura CZ' (e-INFRA LM2018140) provided within the program Projects of Large Research, Development and Innovations Infrastructures.

Funding Information:
We are grateful to L. Zou (KAUST) for technical assistance, Y. Zhou (KAUST) and H.I. Ahmed (KAUST) for discussions on bioinformatics analyses and the KAUST Bioscience Core Lab for sequencing support. We thank Z. Dubská, R. Šperková and J. Weiserová (Institute of Experimental Botany) for the preparation of chromosome samples for flow cytometry, M. Said and P. Cápál (Institute of Experimental Botany) for chromosome 2B sorting and J. Raupp from the Wheat Genetics Resource Center for providing germplasm. We thank J. A. Kolmer from the USDA-ARS Cereal Disease Laboratory, St. Paul, MN, for providing P. triticina isolate MNPSD. We thank P. Lu from IGDB, CAS, for providing TKP sequences for phylogenetic analysis. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award OSR-CRG2018-3768. D.K. was supported by WGRC/IUCRC and NSF (grant 1822162). I.M. received support from the Marie Curie Fellowship grant ‘AEGILWHEAT’ (H2020-MSCA-IF-2016-746253) and from the Hungarian National Research, Development and Innovation Office (K135057). M.K., K.H. and J.D. received support from the ERDF project 'Plants as a tool for sustainable global development' (CZ.02.1.01/0.0/0.0/16_019/0000827). Computational resources were supplied by the project 'e-Infrastruktura CZ' (e-INFRA LM2018140) provided within the program Projects of Large Research, Development and Innovations Infrastructures.

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • Genetics

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