An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by 31P NMR

Wouter R.L. Van Der Star, Cor Dijkema, Pieter De Waard, Cristian Picioreanu, Marc Strous, Mark C.M. Van Loosdrecht

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52 Scopus citations

Abstract

The cytoplasm of anaerobic ammonium oxidizing (anammox) bacteria consists of three compartments separated by membranes. It has been suggested that a proton motive force may be generated over the membrane of the innermost compartment, the "anammoxosome". 31P nuclear magnetic resonance (NMR) spectroscopy was employed to investigate intracellular pH differences in the anammox bacterium Kuenenia stuttgartiensis. With in vivo NMR, spectra were recorded of active, highly concentrated suspensions of K. stuttgartiensis in a wide-bore NMR tube. At different external pH values, two stable and distinct phosphate peaks were apparent in the recorded spectra. These peaks were equivalent with pH values of 7.3 and 6.3 and suggested the presence of a proton motive force over an intracytoplasmic membrane in K.stuttgartiensis. This study provides for the second time-after discovery of acidocalcisome-like compartments in Agrobacterium tumefaciens-evidence for an intracytoplasmic pH gradient in a chemotrophic prokaryotic cell.
Original languageEnglish (US)
Pages (from-to)311-317
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume86
Issue number1
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

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