An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants

Hongchang Cui; Mitchell P. Levesque; Teva Vernoux; Jee W. Jung; Alice J. Paquette; Kimberly L. Gallagher; Jean Y. Wang; Ikram Blilou; Ben Scheres; Philip N. Benfey

doi:10.1126/science.1139531

An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants

Hongchang Cui, Mitchell P. Levesque, Teva Vernoux, Jee W. Jung, Alice J. Paquette, Kimberly L. Gallagher, Jean Y. Wang, Ikram Blilou, Ben Scheres, Philip N. Benfey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

468 Scopus citations

Abstract

Intercellular protein movement plays a critical role in animal and plant development. SHORTROOT (SHR) is a moving transcription factor essential for endodermis specification in the Arabidopsis root. Unlike diffusible animal morphogens, which form a gradient across multiple cell layers, SHR movement is limited to essentially one cell layer. However, the molecular mechanism is unknown. We show that SCARECROW (SCR) blocks SHR movement by sequestering it into the nucleus through protein-protein interaction and a safeguard mechanism that relies on a SHR/SCR-dependent positive feedback loop for SCR transcription. Our studies with SHR and SCR homologs from rice suggest that this mechanism is evolutionarily conserved, providing a plausible explanation why nearly all plants have a single layer of endodermis.

Original language	English (US)
Pages (from-to)	421-425
Number of pages	5
Journal	SCIENCE
Volume	316
Issue number	5823
DOIs	https://doi.org/10.1126/science.1139531
State	Published - Apr 20 2007
Externally published	Yes

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title = "An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants",

abstract = "Intercellular protein movement plays a critical role in animal and plant development. SHORTROOT (SHR) is a moving transcription factor essential for endodermis specification in the Arabidopsis root. Unlike diffusible animal morphogens, which form a gradient across multiple cell layers, SHR movement is limited to essentially one cell layer. However, the molecular mechanism is unknown. We show that SCARECROW (SCR) blocks SHR movement by sequestering it into the nucleus through protein-protein interaction and a safeguard mechanism that relies on a SHR/SCR-dependent positive feedback loop for SCR transcription. Our studies with SHR and SCR homologs from rice suggest that this mechanism is evolutionarily conserved, providing a plausible explanation why nearly all plants have a single layer of endodermis.",

author = "Hongchang Cui and Levesque, {Mitchell P.} and Teva Vernoux and Jung, {Jee W.} and Paquette, {Alice J.} and Gallagher, {Kimberly L.} and Wang, {Jean Y.} and Ikram Blilou and Ben Scheres and Benfey, {Philip N.}",

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T1 - An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants

AU - Cui, Hongchang

AU - Levesque, Mitchell P.

AU - Vernoux, Teva

AU - Jung, Jee W.

AU - Paquette, Alice J.

AU - Gallagher, Kimberly L.

AU - Wang, Jean Y.

AU - Blilou, Ikram

AU - Scheres, Ben

AU - Benfey, Philip N.

PY - 2007/4/20

Y1 - 2007/4/20

N2 - Intercellular protein movement plays a critical role in animal and plant development. SHORTROOT (SHR) is a moving transcription factor essential for endodermis specification in the Arabidopsis root. Unlike diffusible animal morphogens, which form a gradient across multiple cell layers, SHR movement is limited to essentially one cell layer. However, the molecular mechanism is unknown. We show that SCARECROW (SCR) blocks SHR movement by sequestering it into the nucleus through protein-protein interaction and a safeguard mechanism that relies on a SHR/SCR-dependent positive feedback loop for SCR transcription. Our studies with SHR and SCR homologs from rice suggest that this mechanism is evolutionarily conserved, providing a plausible explanation why nearly all plants have a single layer of endodermis.

AB - Intercellular protein movement plays a critical role in animal and plant development. SHORTROOT (SHR) is a moving transcription factor essential for endodermis specification in the Arabidopsis root. Unlike diffusible animal morphogens, which form a gradient across multiple cell layers, SHR movement is limited to essentially one cell layer. However, the molecular mechanism is unknown. We show that SCARECROW (SCR) blocks SHR movement by sequestering it into the nucleus through protein-protein interaction and a safeguard mechanism that relies on a SHR/SCR-dependent positive feedback loop for SCR transcription. Our studies with SHR and SCR homologs from rice suggest that this mechanism is evolutionarily conserved, providing a plausible explanation why nearly all plants have a single layer of endodermis.

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An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants

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