An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase

Patience Chatukuta; Tshegofatso B. Dikobe; David T. Kawadza; Katlego S. Sehlabane; Mutsa M. Takundwa; Aloysius Wong; Chris Gehring; Oziniel Ruzvidzo

doi:10.3390/biom8020015

An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase

Patience Chatukuta, Tshegofatso B. Dikobe, David T. Kawadza, Katlego S. Sehlabane, Mutsa M. Takundwa, Aloysius Wong, Chris Gehring, Oziniel Ruzvidzo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP ^261–379 ) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.

Original language	English (US)
Article number	15
Journal	Biomolecules
Volume	8
Issue number	2
DOIs	https://doi.org/10.3390/biom8020015
State	Published - Jun 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Adenylate cyclase
Arabidopsis thaliana
CAMP
Clathrin assembly
Endocytosis
Pathogen responses

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.3390/biom8020015

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title = "An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase",

abstract = " Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP 261–379 ) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.",

keywords = "Adenylate cyclase, Arabidopsis thaliana, CAMP, Clathrin assembly, Endocytosis, Pathogen responses",

author = "Patience Chatukuta and Dikobe, {Tshegofatso B.} and Kawadza, {David T.} and Sehlabane, {Katlego S.} and Takundwa, {Mutsa M.} and Aloysius Wong and Chris Gehring and Oziniel Ruzvidzo",

note = "Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2018",

month = jun,

doi = "10.3390/biom8020015",

language = "English (US)",

volume = "8",

journal = "Biomolecules",

issn = "2218-273X",

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T1 - An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase

AU - Chatukuta, Patience

AU - Dikobe, Tshegofatso B.

AU - Kawadza, David T.

AU - Sehlabane, Katlego S.

AU - Takundwa, Mutsa M.

AU - Wong, Aloysius

AU - Gehring, Chris

AU - Ruzvidzo, Oziniel

PY - 2018/6

Y1 - 2018/6

N2 - Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP 261–379 ) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.

AB - Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP 261–379 ) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.

KW - Adenylate cyclase

KW - Arabidopsis thaliana

KW - CAMP

KW - Clathrin assembly

KW - Endocytosis

KW - Pathogen responses

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U2 - 10.3390/biom8020015

DO - 10.3390/biom8020015

M3 - Article

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AN - SCOPUS:85044624793

SN - 2218-273X

VL - 8

JO - Biomolecules

JF - Biomolecules

IS - 2

M1 - 15

ER -

An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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