Amyloid-β aggregates cause alterations of astrocytic metabolic phenotype: Impact on neuronal viability

Igor Allaman*, Mathilde Gavillet, Mireille Bélanger, Thierry Laroche, David Viertl, Hilal A. Lashuel, Pierre J. Magistretti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

223 Scopus citations


Amyloid-β(Aβ) peptidesplayakeyrole inthepathogenesisofAlzheimer'sdiseaseandexertvarioustoxic effectsonneurons; however,relatively little isknownabouttheir influenceonglial cells. Astrocytes playapivotal role inbrainhomeostasis,contributing to the regulation of localenergy metabolism and oxidative stress defense, two aspects of importance for neuronal viability and function. In the present study, we explored the effects of Aβ peptides on glucose metabolism in cultured astrocytes. Following Aβ25-35 exposure, we observed an increase in glucose uptake and its various metabolic fates, i.e., glycolysis (coupled to lactate release), tricarboxylic acid cycle, pentose phosphate pathway, and incorporation into glycogen. Aβ increased hydrogen peroxide production as well as glutathione release into the extracellular space without affecting intracellular glutathione content.Acausal link between the effects of Aβ on glucose metabolism and its aggregation and internalization into astrocytes through binding to members of the class A scavenger receptor family could be demonstrated. Using astrocyte-neuron cocultures, we observed that the overall modifications of astrocyte metabolism induced by Aβ impair neuronal viability. The effects of the Aβ25-35 fragment were reproduced by Aβ1-42 but not by Aβ1-40. Finally, the phosphoinositide 3-kinase (PI3-kinase) pathway appears to be crucial in these events since both the changes in glucose utilization and the decrease in neuronal viability are prevented by LY294002, a PI3-kinase inhibitor. This set of observations indicates that Aβ aggregation and internalization into astrocytes profoundly alter their metabolic phenotype with deleterious consequences for neuronal viability.

Original languageEnglish (US)
Pages (from-to)3326-3338
Number of pages13
JournalJournal of Neuroscience
Issue number9
StatePublished - Mar 3 2010
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


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