Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière*, Anca Marian, Tristan Colomb, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.

Original languageEnglish (US)
Pages (from-to)2456-2458
Number of pages3
JournalOptics Letters
Issue number16
StatePublished - Aug 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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