Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière; Anca Marian; Tristan Colomb; Pierre Marquet; Christian Depeursinge

doi:10.1364/OL.32.002456

Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière^*, Anca Marian, Tristan Colomb, Pierre Marquet, Christian Depeursinge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.

Original language	English (US)
Pages (from-to)	2456-2458
Number of pages	3
Journal	OPTICS LETTERS
Volume	32
Issue number	16
DOIs	https://doi.org/10.1364/OL.32.002456
State	Published - Aug 15 2007
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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abstract = "We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.",

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AU - Charrière, Florian

AU - Marian, Anca

AU - Colomb, Tristan

AU - Marquet, Pierre

AU - Depeursinge, Christian

PY - 2007/8/15

Y1 - 2007/8/15

N2 - We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.

AB - We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.

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Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Abstract

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