Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome

Stylianos Fodelianakis, E. A. Antoniou, Francesca Mapelli, Mirko Magagnini, Maria Nikolopoulou, Ramona Marasco, Marta Barbato, Areti Tsiola, I. Tsikopoulou, L. Giaccaglia, Mouna Mahjoubi, Atef Jaouani, R. Amer, Emad I. Hussein, Fuad A. Al-Horani, Fatiha Benzha, Mohamed Blaghen, Hanan Issa Malkawi, Yasser Refaat Abdel-Fattah, Ameur CherifDaniele Daffonchio, Nicolas E. Kalogerakis

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Oil-polluted sediment bioremediation depends on both physicochemical and biological parameters, but the effect of the latter cannot be evaluated without the optimization of the former. We aimed in optimizing the physicochemical parameters related to biodegradation by applying an ex-situ landfarming set-up combined with biostimulation to oil-polluted sediment, in order to determine the added effect of bioaugmentation by four allochthonous oil-degrading bacterial consortia in relation to the degradation efficiency of the indigenous community. We monitored hydrocarbon degradation, sediment ecotoxicity and hydrolytic activity, bacterial population sizes and bacterial community dynamics, characterizing the dominant taxa through time and at each treatment. We observed no significant differences in total degradation, but increased ecotoxicity between the different treatments receiving both biostimulation and bioaugmentation and the biostimulated-only control. Moreover, the added allochthonous bacteria quickly perished and were rarely detected, their addition inducing minimal shifts in community structure although it altered the distribution of the residual hydrocarbons in two treatments. Therefore, we concluded that biodegradation was mostly performed by the autochthonous populations while bioaugmentation, in contrast to biostimulation, did not enhance the remediation process. Our results indicate that when environmental conditions are optimized, the indigenous microbiome at a polluted site will likely outperform any allochthonous consortium.
Original languageEnglish (US)
Pages (from-to)78-86
Number of pages9
JournalJournal of Hazardous Materials
StatePublished - Apr 2015

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was funded by FP-7 PROJECT No. 266473, "Unravelling and exploiting Mediterranean Sea microbial diversity and ecology for xenobiotics' and pollutants' clean up" - ULIXES. The authors would like to thank Prof. Nico Boon for his valuable advice and the five anonymous reviewers for their constructive comments. Francesca Mapelli was supported by Universita degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract "Dote Ricerca").

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Environmental Engineering
  • Health, Toxicology and Mutagenesis
  • Waste Management and Disposal


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