All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.

Hongyun So, Kunwoo Lee, Niren Murthy, Albert P Pisano

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.
Original languageEnglish (US)
Pages (from-to)20693-20699
Number of pages7
JournalACS Applied Materials & Interfaces
Volume6
Issue number23
DOIs
StatePublished - Nov 24 2014
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the grants from the KAUST Global Collaborative Research under the Academic Excellence Alliance program, the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in the Nanotechnology award (HHSN268201000043C), RO1HL096796-02, and RO1AI088023-03. The authors are also grateful for support from Marvell Nanolab and Biomolecular Nanotechnology Center at UC Berkeley where all devices were fabricated. H.S. greatly acknowledges Prof. Young Ho Seo at Kangwon National University in Korea for his valuable discussion.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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