Air-loaded Gas Vesicle Nanoparticles Promote Cell Growth in Three-dimensional Bioprinted Tissue Constructs

Salwa Alshehri; Ram Karan; Sarah Ghalayini; Kowther Kahin; Zainab Khan; Dominik Renn; Sam Mathew; Magnus Rueping; Charlotte A.E. Hauser

doi:10.18063/ijb.v8i3.489

Air-loaded Gas Vesicle Nanoparticles Promote Cell Growth in Three-dimensional Bioprinted Tissue Constructs

Salwa Alshehri, Ram Karan, Sarah Ghalayini, Kowther Kahin, Zainab Khan, Dominik Renn, Sam Mathew, Magnus Rueping^*, Charlotte A.E. Hauser^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Three-dimensional (3D) bioprinting has emerged as a promising method for the engineering of tissues and organs. Still, it faces challenges in its widespread use due to issues with the development of bioink materials and the nutrient diffusion barrier inherent to these scaffold materials. Herein, we introduce a method to promote oxygen diffusion throughout the printed constructs using genetically encoded gas vesicles derived from haloarchaea. These hollow nanostructures are composed of a protein shell that allows gases to permeate freely while excluding the water flow. After printing cells with gas vesicles of various concentrations, the cells were observed to have increased activity and proliferation. These results suggest that air-filled gas vesicles can help overcome the diffusion barrier throughout the 3D bioprinted constructs by increasing oxygen availability to cells within the center of the construct. The biodegradable nature of the gas vesicle proteins combined with our promising results encourage their potential use as oxygen-promoting materials in biological samples.

Original language	English (US)
Pages (from-to)	69-81
Number of pages	13
Journal	International Journal of Bioprinting
Volume	8
Issue number	3
DOIs	https://doi.org/10.18063/ijb.v8i3.489
State	Published - 2022

Bibliographical note

Funding Information:
The authors thank Dr. Thorsten Allers for generously providing us with H. volcanii H1895 and its corresponding vector pTA963. We thank the research intern, Alvin Huang, for protein nanoparticle production and purification assistance. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology and University of Jeddah.

Publisher Copyright:
© 2022. Author(s). This is an Open-Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

Gas vesicles
Halobacterium
Haloferax
Three-dimensional bioprinting
Tissue engineering

ASJC Scopus subject areas

Biotechnology
Materials Science (miscellaneous)
Industrial and Manufacturing Engineering

Access to Document

10.18063/ijb.v8i3.489

Cite this

@article{812cfd6fbbb64733ad10a429f5e95c36,

title = "Air-loaded Gas Vesicle Nanoparticles Promote Cell Growth in Three-dimensional Bioprinted Tissue Constructs",

abstract = "Three-dimensional (3D) bioprinting has emerged as a promising method for the engineering of tissues and organs. Still, it faces challenges in its widespread use due to issues with the development of bioink materials and the nutrient diffusion barrier inherent to these scaffold materials. Herein, we introduce a method to promote oxygen diffusion throughout the printed constructs using genetically encoded gas vesicles derived from haloarchaea. These hollow nanostructures are composed of a protein shell that allows gases to permeate freely while excluding the water flow. After printing cells with gas vesicles of various concentrations, the cells were observed to have increased activity and proliferation. These results suggest that air-filled gas vesicles can help overcome the diffusion barrier throughout the 3D bioprinted constructs by increasing oxygen availability to cells within the center of the construct. The biodegradable nature of the gas vesicle proteins combined with our promising results encourage their potential use as oxygen-promoting materials in biological samples.",

keywords = "Gas vesicles, Halobacterium, Haloferax, Three-dimensional bioprinting, Tissue engineering",

author = "Salwa Alshehri and Ram Karan and Sarah Ghalayini and Kowther Kahin and Zainab Khan and Dominik Renn and Sam Mathew and Magnus Rueping and Hauser, {Charlotte A.E.}",

note = "Funding Information: The authors thank Dr. Thorsten Allers for generously providing us with H. volcanii H1895 and its corresponding vector pTA963. We thank the research intern, Alvin Huang, for protein nanoparticle production and purification assistance. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology and University of Jeddah. Publisher Copyright: {\textcopyright} 2022. Author(s). This is an Open-Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.",

year = "2022",

doi = "10.18063/ijb.v8i3.489",

language = "English (US)",

volume = "8",

pages = "69--81",

journal = "International Journal of Bioprinting",

issn = "2424-7723",

publisher = "AccScience Publishing",

number = "3",

}

TY - JOUR

T1 - Air-loaded Gas Vesicle Nanoparticles Promote Cell Growth in Three-dimensional Bioprinted Tissue Constructs

AU - Alshehri, Salwa

AU - Karan, Ram

AU - Ghalayini, Sarah

AU - Kahin, Kowther

AU - Khan, Zainab

AU - Renn, Dominik

AU - Mathew, Sam

AU - Rueping, Magnus

AU - Hauser, Charlotte A.E.

N1 - Funding Information: The authors thank Dr. Thorsten Allers for generously providing us with H. volcanii H1895 and its corresponding vector pTA963. We thank the research intern, Alvin Huang, for protein nanoparticle production and purification assistance. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology and University of Jeddah. Publisher Copyright: © 2022. Author(s). This is an Open-Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2022

Y1 - 2022

N2 - Three-dimensional (3D) bioprinting has emerged as a promising method for the engineering of tissues and organs. Still, it faces challenges in its widespread use due to issues with the development of bioink materials and the nutrient diffusion barrier inherent to these scaffold materials. Herein, we introduce a method to promote oxygen diffusion throughout the printed constructs using genetically encoded gas vesicles derived from haloarchaea. These hollow nanostructures are composed of a protein shell that allows gases to permeate freely while excluding the water flow. After printing cells with gas vesicles of various concentrations, the cells were observed to have increased activity and proliferation. These results suggest that air-filled gas vesicles can help overcome the diffusion barrier throughout the 3D bioprinted constructs by increasing oxygen availability to cells within the center of the construct. The biodegradable nature of the gas vesicle proteins combined with our promising results encourage their potential use as oxygen-promoting materials in biological samples.

AB - Three-dimensional (3D) bioprinting has emerged as a promising method for the engineering of tissues and organs. Still, it faces challenges in its widespread use due to issues with the development of bioink materials and the nutrient diffusion barrier inherent to these scaffold materials. Herein, we introduce a method to promote oxygen diffusion throughout the printed constructs using genetically encoded gas vesicles derived from haloarchaea. These hollow nanostructures are composed of a protein shell that allows gases to permeate freely while excluding the water flow. After printing cells with gas vesicles of various concentrations, the cells were observed to have increased activity and proliferation. These results suggest that air-filled gas vesicles can help overcome the diffusion barrier throughout the 3D bioprinted constructs by increasing oxygen availability to cells within the center of the construct. The biodegradable nature of the gas vesicle proteins combined with our promising results encourage their potential use as oxygen-promoting materials in biological samples.

KW - Gas vesicles

KW - Halobacterium

KW - Haloferax

KW - Three-dimensional bioprinting

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85131440144&partnerID=8YFLogxK

U2 - 10.18063/ijb.v8i3.489

DO - 10.18063/ijb.v8i3.489

M3 - Article

C2 - 36105129

AN - SCOPUS:85131440144

SN - 2424-7723

VL - 8

SP - 69

EP - 81

JO - International Journal of Bioprinting

JF - International Journal of Bioprinting

IS - 3

ER -

Air-loaded Gas Vesicle Nanoparticles Promote Cell Growth in Three-dimensional Bioprinted Tissue Constructs

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this