Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming

Bodor Fallatah; Muhammad Shuaib; Sabir Adroub; Andreu Paytuví-Gallart; Francesco Della Valle; Seba S. Nadeef; Chiara Lanzuolo; Valerio Orlando

doi:10.1016/j.celrep.2021.110066

Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming

Bodor Fallatah, Muhammad Shuaib, Sabir Adroub, Andreu Paytuví-Gallart, Francesco Della Valle, Seba S. Nadeef, Chiara Lanzuolo, Valerio Orlando

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The role of chromatin-associated RNAi components in the nucleus of mammalian cells and in particular in the context of developmental programs remains to be elucidated. Here, we investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation. We show that Ago1 is required for activation of the myogenic program by supporting chromatin modification mediated by developmental enhancer activation. Mechanistically, we demonstrate that Ago1 directly controls global H3K27 acetylation (H3K27ac) by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction, a key step in enhancer-driven gene activation. In particular, we show that Ago1 is specifically required for myogenic differentiation 1 (MyoD) and downstream myogenic gene activation, whereas its depletion leads to failure of CBP acetyltransferase activation and blocking of the myogenic program. Our work establishes a role of the mammalian enhancer-associated RNAi component Ago1 in epigenome regulation and activation of developmental programs.

Original language	English (US)
Pages (from-to)	110066
Journal	Cell Reports
Volume	37
Issue number	9
DOIs	https://doi.org/10.1016/j.celrep.2021.110066
State	Published - Nov 2021

Bibliographical note

KAUST Repository Item: Exported on 2022-01-27
Acknowledgements: We are grateful to Riccardo Aiese Cigliano and his team (Sequentia Biotech) for training B.F. on ChIP-seq analysis and for excellent support; Yao Wu (scientific illustrator at KAUST) for help in drawing the graphical abstract; and the KAUST Bioscience Core Lab for providing sequencing and imaging facilities. The work was supported by King Abdullah University of Science and Technology (KAUST) to V.O.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2021.110066

https://repository.kaust.edu.sa/bitstream/10754/673856/1/1-s2.0-S2211124721015527-main.pdf

Cite this

@article{fe24b5c1cb3b4b4db4a21829fe73dfa7,

title = "Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming",

abstract = "The role of chromatin-associated RNAi components in the nucleus of mammalian cells and in particular in the context of developmental programs remains to be elucidated. Here, we investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation. We show that Ago1 is required for activation of the myogenic program by supporting chromatin modification mediated by developmental enhancer activation. Mechanistically, we demonstrate that Ago1 directly controls global H3K27 acetylation (H3K27ac) by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction, a key step in enhancer-driven gene activation. In particular, we show that Ago1 is specifically required for myogenic differentiation 1 (MyoD) and downstream myogenic gene activation, whereas its depletion leads to failure of CBP acetyltransferase activation and blocking of the myogenic program. Our work establishes a role of the mammalian enhancer-associated RNAi component Ago1 in epigenome regulation and activation of developmental programs.",

author = "Bodor Fallatah and Muhammad Shuaib and Sabir Adroub and Andreu Paytuv{\'i}-Gallart and {Della Valle}, Francesco and Nadeef, {Seba S.} and Chiara Lanzuolo and Valerio Orlando",

note = "KAUST Repository Item: Exported on 2022-01-27 Acknowledgements: We are grateful to Riccardo Aiese Cigliano and his team (Sequentia Biotech) for training B.F. on ChIP-seq analysis and for excellent support; Yao Wu (scientific illustrator at KAUST) for help in drawing the graphical abstract; and the KAUST Bioscience Core Lab for providing sequencing and imaging facilities. The work was supported by King Abdullah University of Science and Technology (KAUST) to V.O.",

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AU - Shuaib, Muhammad

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AU - Paytuví-Gallart, Andreu

AU - Della Valle, Francesco

AU - Nadeef, Seba S.

AU - Lanzuolo, Chiara

AU - Orlando, Valerio

N1 - KAUST Repository Item: Exported on 2022-01-27 Acknowledgements: We are grateful to Riccardo Aiese Cigliano and his team (Sequentia Biotech) for training B.F. on ChIP-seq analysis and for excellent support; Yao Wu (scientific illustrator at KAUST) for help in drawing the graphical abstract; and the KAUST Bioscience Core Lab for providing sequencing and imaging facilities. The work was supported by King Abdullah University of Science and Technology (KAUST) to V.O.

PY - 2021/11

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N2 - The role of chromatin-associated RNAi components in the nucleus of mammalian cells and in particular in the context of developmental programs remains to be elucidated. Here, we investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation. We show that Ago1 is required for activation of the myogenic program by supporting chromatin modification mediated by developmental enhancer activation. Mechanistically, we demonstrate that Ago1 directly controls global H3K27 acetylation (H3K27ac) by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction, a key step in enhancer-driven gene activation. In particular, we show that Ago1 is specifically required for myogenic differentiation 1 (MyoD) and downstream myogenic gene activation, whereas its depletion leads to failure of CBP acetyltransferase activation and blocking of the myogenic program. Our work establishes a role of the mammalian enhancer-associated RNAi component Ago1 in epigenome regulation and activation of developmental programs.

AB - The role of chromatin-associated RNAi components in the nucleus of mammalian cells and in particular in the context of developmental programs remains to be elucidated. Here, we investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation. We show that Ago1 is required for activation of the myogenic program by supporting chromatin modification mediated by developmental enhancer activation. Mechanistically, we demonstrate that Ago1 directly controls global H3K27 acetylation (H3K27ac) by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction, a key step in enhancer-driven gene activation. In particular, we show that Ago1 is specifically required for myogenic differentiation 1 (MyoD) and downstream myogenic gene activation, whereas its depletion leads to failure of CBP acetyltransferase activation and blocking of the myogenic program. Our work establishes a role of the mammalian enhancer-associated RNAi component Ago1 in epigenome regulation and activation of developmental programs.

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JO - Cell Reports

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Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming

Abstract

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