Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming

Bodor Fallatah, Muhammad Shuaib, Sabir Adroub, Andreu Paytuví-Gallart, Francesco Della Valle, Seba S. Nadeef, Chiara Lanzuolo, Valerio Orlando

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The role of chromatin-associated RNAi components in the nucleus of mammalian cells and in particular in the context of developmental programs remains to be elucidated. Here, we investigate the function of nuclear Argonaute 1 (Ago1) in gene expression regulation during skeletal muscle differentiation. We show that Ago1 is required for activation of the myogenic program by supporting chromatin modification mediated by developmental enhancer activation. Mechanistically, we demonstrate that Ago1 directly controls global H3K27 acetylation (H3K27ac) by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction, a key step in enhancer-driven gene activation. In particular, we show that Ago1 is specifically required for myogenic differentiation 1 (MyoD) and downstream myogenic gene activation, whereas its depletion leads to failure of CBP acetyltransferase activation and blocking of the myogenic program. Our work establishes a role of the mammalian enhancer-associated RNAi component Ago1 in epigenome regulation and activation of developmental programs.
Original languageEnglish (US)
Pages (from-to)110066
JournalCell Reports
Volume37
Issue number9
DOIs
StatePublished - Nov 2021

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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