Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

Research output: Contribution to journalArticlepeer-review


We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.
Original languageEnglish (US)
JournalmicroPublication biology
StatePublished - Sep 14 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-10-03
Acknowledged KAUST grant number(s): CRG10 URF/1/4705-01-01
Acknowledgements: KAUST intramural funding and an OSR competitive research grant (CRG10 URF/1/4705-01-01). We thank the CGC, which is funded by the NIH Office of Research Infrastructure Programs (grant no. P40 OD010440) and WormBase for data and literature curation.


Dive into the research topics of 'Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant'. Together they form a unique fingerprint.

Cite this