Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

Monika Priyadarshini; Sarah Al-Harbi; Christian Frøkjær-Jensen

Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

Monika Priyadarshini, Sarah Al-Harbi, Christian Frøkjær-Jensen

Research output: Contribution to journal › Article › peer-review

Abstract

We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.

Original language	English (US)
Journal	microPublication biology
State	Published - Sep 14 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-10-03
Acknowledged KAUST grant number(s): CRG10 URF/1/4705-01-01
Acknowledgements: KAUST intramural funding and an OSR competitive research grant (CRG10 URF/1/4705-01-01). We thank the CGC, which is funded by the NIH Office of Research Infrastructure Programs (grant no. P40 OD010440) and WormBase for data and literature curation.

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title = "Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant",

abstract = "We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.",

author = "Monika Priyadarshini and Sarah Al-Harbi and Christian Fr{\o}kj{\ae}r-Jensen",

note = "KAUST Repository Item: Exported on 2022-10-03 Acknowledged KAUST grant number(s): CRG10 URF/1/4705-01-01 Acknowledgements: KAUST intramural funding and an OSR competitive research grant (CRG10 URF/1/4705-01-01). We thank the CGC, which is funded by the NIH Office of Research Infrastructure Programs (grant no. P40 OD010440) and WormBase for data and literature curation.",

year = "2022",

month = sep,

day = "14",

language = "English (US)",

journal = "microPublication biology",

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TY - JOUR

T1 - Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

AU - Priyadarshini, Monika

AU - Al-Harbi, Sarah

AU - Frøkjær-Jensen, Christian

N1 - KAUST Repository Item: Exported on 2022-10-03 Acknowledged KAUST grant number(s): CRG10 URF/1/4705-01-01 Acknowledgements: KAUST intramural funding and an OSR competitive research grant (CRG10 URF/1/4705-01-01). We thank the CGC, which is funded by the NIH Office of Research Infrastructure Programs (grant no. P40 OD010440) and WormBase for data and literature curation.

PY - 2022/9/14

Y1 - 2022/9/14

N2 - We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.

AB - We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.

UR - http://hdl.handle.net/10754/681759

M3 - Article

SN - 2578-9430

JO - microPublication biology

JF - microPublication biology

ER -

Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

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