Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Jessica L. Cohen; Stephanie Schubert; Peter R. Wich; Lina Cui; Joel A. Cohen; Justin L. Mynar; Jean M.J. Fréchet

doi:10.1021/bc100542r

Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Jessica L. Cohen, Stephanie Schubert, Peter R. Wich, Lina Cui, Joel A. Cohen, Justin L. Mynar, Jean M.J. Fréchet

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

Original language	English (US)
Pages (from-to)	1056-1065
Number of pages	10
Journal	Bioconjugate Chemistry
Volume	22
Issue number	6
DOIs	https://doi.org/10.1021/bc100542r
State	Published - Jun 15 2011

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

Access to Document

10.1021/bc100542r

Cite this

@article{e1df9140a7a340bfbaf7c77d7075a1f0,

title = "Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics",

abstract = "We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.",

author = "Cohen, {Jessica L.} and Stephanie Schubert and Wich, {Peter R.} and Lina Cui and Cohen, {Joel A.} and Mynar, {Justin L.} and Fr{\'e}chet, {Jean M.J.}",

year = "2011",

month = jun,

day = "15",

doi = "10.1021/bc100542r",

language = "English (US)",

volume = "22",

pages = "1056--1065",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

AU - Cohen, Jessica L.

AU - Schubert, Stephanie

AU - Wich, Peter R.

AU - Cui, Lina

AU - Cohen, Joel A.

AU - Mynar, Justin L.

AU - Fréchet, Jean M.J.

PY - 2011/6/15

Y1 - 2011/6/15

N2 - We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

AB - We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

UR - http://www.scopus.com/inward/record.url?scp=79959201542&partnerID=8YFLogxK

U2 - 10.1021/bc100542r

DO - 10.1021/bc100542r

M3 - Article

C2 - 21539393

AN - SCOPUS:79959201542

SN - 1043-1802

VL - 22

SP - 1056

EP - 1065

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 6

ER -

Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this