Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics

Jessica L. Cohen, Stephanie Schubert, Peter R. Wich, Lina Cui, Joel A. Cohen, Justin L. Mynar, Jean M.J. Fréchet

    Research output: Contribution to journalArticlepeer-review

    141 Scopus citations

    Abstract

    We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

    Original languageEnglish (US)
    Pages (from-to)1056-1065
    Number of pages10
    JournalBioconjugate Chemistry
    Volume22
    Issue number6
    DOIs
    StatePublished - Jun 15 2011

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Pharmacology
    • Pharmaceutical Science
    • Organic Chemistry

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