The RIKEN expression array database (READ) provides comprehensive gene expression data for the mouse, which were obtained as relative values from microarray double-staining experiments with E17.5 mRNA as common reference. To assign absolute expression values for mouse transcripts within READ, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. Newly assigned values within the READ database were validated by comparison to expression data from serial analysis of gene expression, CAGE and EST experiments. These experiments confirmed the great significance of the absolute expression values within the improved READ database.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Feb 13 2004|
Bibliographical noteFunding Information:
We thank A. Hasegawa for database management and bioinformatic analysis, also Y. Mitsuiki for secretarial support. This work was supported by Research Grants for the RIKEN Genome Exploration Research Project from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government to Y.H., Research Grant for Advanced and Innovational Research Program in Life Science from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government to J.K. and Presidential Research Grant for Intersystem Collaboration of RIKEN to J.K. R.K. is the recipient of an INCO-JAPAN fellowship from the European Union.
- Absolute value
- Cap analysis of gene expression
- Expressed sequence tag count
- Gene expression
- Serial analysis of gene expression
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
- Cell Biology