A wheat kinase and immune receptor form host-specificity barriers against the blast fungus

Sanu Arora; Andrew Steed; Rachel Goddard; Kumar Gaurav; Tom O’Hara; Adam Schoen; Nidhi Rawat; Ahmed F. Elkot; Andrey V. Korolev; Catherine Chinoy; Martha H. Nicholson; Soichiro Asuke; Rea Antoniou-Kourounioti; Burkhard Steuernagel; Guotai Yu; Rajani Awal; Macarena Forner-Martínez; Luzie Wingen; Erin Baggs; Jonathan Clarke; Diane G.O. Saunders; Ksenia V. Krasileva; Yukio Tosa; Jonathan D.G. Jones; Vijay K. Tiwari; Brande B.H. Wulff; Paul Nicholson

doi:10.1038/s41477-023-01357-5

A wheat kinase and immune receptor form host-specificity barriers against the blast fungus

Sanu Arora, Andrew Steed, Rachel Goddard, Kumar Gaurav, Tom O’Hara, Adam Schoen, Nidhi Rawat, Ahmed F. Elkot, Andrey V. Korolev, Catherine Chinoy, Martha H. Nicholson, Soichiro Asuke, Rea Antoniou-Kourounioti, Burkhard Steuernagel, Guotai Yu, Rajani Awal, Macarena Forner-Martínez, Luzie Wingen, Erin Baggs, Jonathan ClarkeDiane G.O. Saunders, Ksenia V. Krasileva, Yukio Tosa, Jonathan D.G. Jones, Vijay K. Tiwari, Brande B.H. Wulff^*, Paul Nicholson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. Here we show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes will enable study of the molecular interaction between pathogen effector and host resistance genes.

Original language	English (US)
Pages (from-to)	385-392
Number of pages	8
Journal	Nature Plants
Volume	9
Issue number	3
DOIs	https://doi.org/10.1038/s41477-023-01357-5
State	Published - Mar 2023

Bibliographical note

Funding Information:
The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B.

Funding Information:
The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B.

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

Plant Science

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10.1038/s41477-023-01357-5

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Arora, S., Steed, A., Goddard, R., Gaurav, K., O’Hara, T., Schoen, A., Rawat, N., Elkot, A. F., Korolev, A. V., Chinoy, C., Nicholson, M. H., Asuke, S., Antoniou-Kourounioti, R., Steuernagel, B., Yu, G., Awal, R., Forner-Martínez, M., Wingen, L., Baggs, E., ... Nicholson, P. (2023). A wheat kinase and immune receptor form host-specificity barriers against the blast fungus. Nature Plants, 9(3), 385-392. https://doi.org/10.1038/s41477-023-01357-5

@article{19350a57791c4ca1902821a5733afe72,

title = "A wheat kinase and immune receptor form host-specificity barriers against the blast fungus",

abstract = "Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. Here we show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes will enable study of the molecular interaction between pathogen effector and host resistance genes.",

author = "Sanu Arora and Andrew Steed and Rachel Goddard and Kumar Gaurav and Tom O{\textquoteright}Hara and Adam Schoen and Nidhi Rawat and Elkot, {Ahmed F.} and Korolev, {Andrey V.} and Catherine Chinoy and Nicholson, {Martha H.} and Soichiro Asuke and Rea Antoniou-Kourounioti and Burkhard Steuernagel and Guotai Yu and Rajani Awal and Macarena Forner-Mart{\'i}nez and Luzie Wingen and Erin Baggs and Jonathan Clarke and Saunders, {Diane G.O.} and Krasileva, {Ksenia V.} and Yukio Tosa and Jones, {Jonathan D.G.} and Tiwari, {Vijay K.} and Wulff, {Brande B.H.} and Paul Nicholson",

note = "Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = mar,

doi = "10.1038/s41477-023-01357-5",

language = "English (US)",

volume = "9",

pages = "385--392",

journal = "Nature Plants",

issn = "2055-026X",

publisher = "Nature Publishing Group",

number = "3",

}

Arora, S, Steed, A, Goddard, R, Gaurav, K, O’Hara, T, Schoen, A, Rawat, N, Elkot, AF, Korolev, AV, Chinoy, C, Nicholson, MH, Asuke, S, Antoniou-Kourounioti, R, Steuernagel, B, Yu, G, Awal, R, Forner-Martínez, M, Wingen, L, Baggs, E, Clarke, J, Saunders, DGO, Krasileva, KV, Tosa, Y, Jones, JDG, Tiwari, VK, Wulff, BBH & Nicholson, P 2023, 'A wheat kinase and immune receptor form host-specificity barriers against the blast fungus', Nature Plants, vol. 9, no. 3, pp. 385-392. https://doi.org/10.1038/s41477-023-01357-5

TY - JOUR

T1 - A wheat kinase and immune receptor form host-specificity barriers against the blast fungus

AU - Arora, Sanu

AU - Steed, Andrew

AU - Goddard, Rachel

AU - Gaurav, Kumar

AU - O’Hara, Tom

AU - Schoen, Adam

AU - Rawat, Nidhi

AU - Elkot, Ahmed F.

AU - Korolev, Andrey V.

AU - Chinoy, Catherine

AU - Nicholson, Martha H.

AU - Asuke, Soichiro

AU - Antoniou-Kourounioti, Rea

AU - Steuernagel, Burkhard

AU - Yu, Guotai

AU - Awal, Rajani

AU - Forner-Martínez, Macarena

AU - Wingen, Luzie

AU - Baggs, Erin

AU - Clarke, Jonathan

AU - Saunders, Diane G.O.

AU - Krasileva, Ksenia V.

AU - Tosa, Yukio

AU - Jones, Jonathan D.G.

AU - Tiwari, Vijay K.

AU - Wulff, Brande B.H.

AU - Nicholson, Paul

N1 - Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. Funding Information: The high-performance computing resources and services used in this work were supported by the Norwich Bioscience Institutes Partnership (NBIP) Computing infrastructure for Science (CiS) group alongside the Earlham Institute (EI) scientific computing group. We are grateful to the John Innes Centre (JIC) Horticultural Services for plant husbandry; EI for providing open access to the Kronos genome; R. Goram at the JIC genotyping platform for the KASP genotyping. This research was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. and P.N. (BBS/E/J/000PR9780); a John Innes Centre Institute Strategic Grant to B.B.H.W.; Science, Technology & Innovation Funding Authority (STDF), Egypt-UK Newton-Mosharafa Institutional Links award, Project ID (30718) to A.F.E. and B.B.H.W.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the Two Blades Foundation; and the Gatsby Charitable Foundation to JDGJ; National Science Foundation (award number 1943155) and USDA NIFA (award numbers 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T.; European Research Commission grant (ERC-2016-STG-716233-MIREDI) to K.V.K. and BBSRC Norwich Research Park Doctoral Training Grant (BB/M011216/1) for supporting E.B. Publisher Copyright: © 2023, The Author(s).

PY - 2023/3

Y1 - 2023/3

N2 - Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. Here we show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes will enable study of the molecular interaction between pathogen effector and host resistance genes.

AB - Since emerging in Brazil in 1985, wheat blast has spread throughout South America and recently appeared in Bangladesh and Zambia. Here we show that two wheat resistance genes, Rwt3 and Rwt4, acting as host-specificity barriers against non-Triticum blast pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor and a tandem kinase, respectively. Molecular isolation of these genes will enable study of the molecular interaction between pathogen effector and host resistance genes.

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U2 - 10.1038/s41477-023-01357-5

DO - 10.1038/s41477-023-01357-5

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AN - SCOPUS:85148217264

SN - 2055-026X

VL - 9

SP - 385

EP - 392

JO - Nature Plants

JF - Nature Plants

IS - 3

ER -

A wheat kinase and immune receptor form host-specificity barriers against the blast fungus

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