A temporary marker for biological applications

M. Sameiro, T. Gonçalves, Hernâni L.S. Maia

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Having in mind the development of new colour labelled amino acid derivatives, a carboxyl azo dye was coupled to amino acid esters to give the corresponding orange N-acyl derivatives, which were in turn further acylated at their N-terminus with Boc for investigation of the conditions of possible cleavage of the chromophore by electrolysis or with nucleophiles. While difficulties were met with electrolysis owing to competitive reduction of the azo group, cleavage with N,N-diethylaminoethylamine (DEAEA) gave satisfactory results. This allows the use of the chromophore as a temporary marker.

Original languageEnglish (US)
Pages (from-to)7775-7777
Number of pages3
JournalTetrahedron Letters
Issue number44
StatePublished - Oct 29 2001

Bibliographical note

Funding Information:
We thank the Foundation for Science and Technology (Portugal) for financial support to the Institute of Biotechnology and Fine Chemistry (University of Minho).


  • Acylation
  • Amino acid
  • Azo compounds
  • Cleavage reactions
  • Dyes

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry


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