A single NLR gene confers resistance to leaf and stripe rust in wheat

Davinder Sharma; Raz Avni; Juan Gutierrez-Gonzalez; Rakesh Kumar; Hanan Sela; Manas Ranjan Prusty; Arava Shatil-Cohen; István Molnár; Kateřina Holušová; Mahmoud Said; Jaroslav Doležel; Eitan Millet; Sofia Khazan-Kost; Udi Landau; Gerit Bethke; Or Sharon; Smadar Ezrati; Moshe Ronen; Oxana Maatuk; Tamar Eilam; Jacob Manisterski; Pnina Ben-Yehuda; Yehoshua Anikster; Oadi Matny; Brian J. Steffenson; Martin Mascher; Helen J. Brabham; Matthew J. Moscou; Yong Liang; Guotai Yu; Brande B.H. Wulff; Gary Muehlbauer; Anna Minz-Dub; Amir Sharon

doi:10.1038/s41467-024-54068-6

A single NLR gene confers resistance to leaf and stripe rust in wheat

Davinder Sharma, Raz Avni, Juan Gutierrez-Gonzalez, Rakesh Kumar, Hanan Sela, Manas Ranjan Prusty, Arava Shatil-Cohen, István Molnár, Kateřina Holušová, Mahmoud Said, Jaroslav Doležel, Eitan Millet, Sofia Khazan-Kost, Udi Landau, Gerit Bethke, Or Sharon, Smadar Ezrati, Moshe Ronen, Oxana Maatuk, Tamar EilamJacob Manisterski, Pnina Ben-Yehuda, Yehoshua Anikster, Oadi Matny, Brian J. Steffenson, Martin Mascher, Helen J. Brabham, Matthew J. Moscou, Yong Liang, Guotai Yu, Brande B.H. Wulff, Gary Muehlbauer^*, Anna Minz-Dub^*, Amir Sharon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.

Original language	English (US)
Article number	9925
Journal	Nature Communications
Volume	15
Issue number	1
DOIs	https://doi.org/10.1038/s41467-024-54068-6
State	Published - Dec 2024

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Sharma, D., Avni, R., Gutierrez-Gonzalez, J., Kumar, R., Sela, H., Prusty, M. R., Shatil-Cohen, A., Molnár, I., Holušová, K., Said, M., Doležel, J., Millet, E., Khazan-Kost, S., Landau, U., Bethke, G., Sharon, O., Ezrati, S., Ronen, M., Maatuk, O., ... Sharon, A. (2024). A single NLR gene confers resistance to leaf and stripe rust in wheat. Nature Communications, 15(1), Article 9925. https://doi.org/10.1038/s41467-024-54068-6

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title = "A single NLR gene confers resistance to leaf and stripe rust in wheat",

abstract = "Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.",

author = "Davinder Sharma and Raz Avni and Juan Gutierrez-Gonzalez and Rakesh Kumar and Hanan Sela and Prusty, {Manas Ranjan} and Arava Shatil-Cohen and Istv{\'a}n Moln{\'a}r and Kate{\v r}ina Holu{\v s}ov{\'a} and Mahmoud Said and Jaroslav Dole{\v z}el and Eitan Millet and Sofia Khazan-Kost and Udi Landau and Gerit Bethke and Or Sharon and Smadar Ezrati and Moshe Ronen and Oxana Maatuk and Tamar Eilam and Jacob Manisterski and Pnina Ben-Yehuda and Yehoshua Anikster and Oadi Matny and Steffenson, {Brian J.} and Martin Mascher and Brabham, {Helen J.} and Moscou, {Matthew J.} and Yong Liang and Guotai Yu and Wulff, {Brande B.H.} and Gary Muehlbauer and Anna Minz-Dub and Amir Sharon",

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doi = "10.1038/s41467-024-54068-6",

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Sharma, D, Avni, R, Gutierrez-Gonzalez, J, Kumar, R, Sela, H, Prusty, MR, Shatil-Cohen, A, Molnár, I, Holušová, K, Said, M, Doležel, J, Millet, E, Khazan-Kost, S, Landau, U, Bethke, G, Sharon, O, Ezrati, S, Ronen, M, Maatuk, O, Eilam, T, Manisterski, J, Ben-Yehuda, P, Anikster, Y, Matny, O, Steffenson, BJ, Mascher, M, Brabham, HJ, Moscou, MJ, Liang, Y, Yu, G , Wulff, BBH, Muehlbauer, G, Minz-Dub, A & Sharon, A 2024, 'A single NLR gene confers resistance to leaf and stripe rust in wheat', Nature Communications, vol. 15, no. 1, 9925. https://doi.org/10.1038/s41467-024-54068-6

TY - JOUR

T1 - A single NLR gene confers resistance to leaf and stripe rust in wheat

AU - Sharma, Davinder

AU - Avni, Raz

AU - Gutierrez-Gonzalez, Juan

AU - Kumar, Rakesh

AU - Sela, Hanan

AU - Prusty, Manas Ranjan

AU - Shatil-Cohen, Arava

AU - Molnár, István

AU - Holušová, Kateřina

AU - Said, Mahmoud

AU - Doležel, Jaroslav

AU - Millet, Eitan

AU - Khazan-Kost, Sofia

AU - Landau, Udi

AU - Bethke, Gerit

AU - Sharon, Or

AU - Ezrati, Smadar

AU - Ronen, Moshe

AU - Maatuk, Oxana

AU - Eilam, Tamar

AU - Manisterski, Jacob

AU - Ben-Yehuda, Pnina

AU - Anikster, Yehoshua

AU - Matny, Oadi

AU - Steffenson, Brian J.

AU - Mascher, Martin

AU - Brabham, Helen J.

AU - Moscou, Matthew J.

AU - Liang, Yong

AU - Yu, Guotai

AU - Wulff, Brande B.H.

AU - Muehlbauer, Gary

AU - Minz-Dub, Anna

AU - Sharon, Amir

PY - 2024/12

Y1 - 2024/12

N2 - Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.

AB - Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.

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U2 - 10.1038/s41467-024-54068-6

DO - 10.1038/s41467-024-54068-6

M3 - Article

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AN - SCOPUS:85209354924

SN - 2041-1723

VL - 15

JO - Nature Communications

JF - Nature Communications

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M1 - 9925

ER -

A single NLR gene confers resistance to leaf and stripe rust in wheat

Abstract

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