A simple method of fabricating mask-free microfluidic devices for biological analysis.

Xin Yi, Rimantas Kodzius, Xiuqing Gong, Kang Xiao, Weijia Wen

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


We report a simple, low-cost, rapid, and mask-free method to fabricate two-dimensional (2D) and three-dimensional (3D) microfluidic chip for biological analysis researches. In this fabrication process, a laser system is used to cut through paper to form intricate patterns and differently configured channels for specific purposes. Bonded with cyanoacrylate-based resin, the prepared paper sheet is sandwiched between glass slides (hydrophilic) or polymer-based plates (hydrophobic) to obtain a multilayer structure. In order to examine the chip's biocompatibility and applicability, protein concentration was measured while DNA capillary electrophoresis was carried out, and both of them show positive results. With the utilization of direct laser cutting and one-step gas-sacrificing techniques, the whole fabrication processes for complicated 2D and 3D microfluidic devices are shorten into several minutes which make it a good alternative of poly(dimethylsiloxane) microfluidic chips used in biological analysis researches.
Original languageEnglish (US)
Pages (from-to)036503
Issue number3
StatePublished - Sep 7 2010

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040
Acknowledgements: Award No. SA-C0040/U.K.-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong RGC Grant No. HKUST 603608; Nanoscience and Nanotechnology Program at HKUST


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