A simple method for isolation of megabase DNA from cotton

Xinping Zhao; Hong Bin Zhang; Rod A. Wing; Andrew H. Paterson

doi:10.1007/BF02668372

A simple method for isolation of megabase DNA from cotton

Xinping Zhao, Hong Bin Zhang, Rod A. Wing, Andrew H. Paterson

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. © 1994 Kluwer Academic Publishers.

Original language	English (US)
Journal	Plant Molecular Biology Reporter
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/BF02668372
State	Published - Jun 1 1994
Externally published	Yes

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Generated from Scopus record by KAUST IRTS on 2019-11-20

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10.1007/BF02668372

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title = "A simple method for isolation of megabase DNA from cotton",

abstract = "A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. {\textcopyright} 1994 Kluwer Academic Publishers.",

author = "Xinping Zhao and Zhang, {Hong Bin} and Wing, {Rod A.} and Paterson, {Andrew H.}",

note = "Generated from Scopus record by KAUST IRTS on 2019-11-20",

year = "1994",

month = jun,

day = "1",

doi = "10.1007/BF02668372",

language = "English (US)",

volume = "12",

journal = "Plant Molecular Biology Reporter",

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AU - Zhao, Xinping

AU - Zhang, Hong Bin

AU - Wing, Rod A.

AU - Paterson, Andrew H.

N1 - Generated from Scopus record by KAUST IRTS on 2019-11-20

PY - 1994/6/1

Y1 - 1994/6/1

N2 - A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. © 1994 Kluwer Academic Publishers.

AB - A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. © 1994 Kluwer Academic Publishers.

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U2 - 10.1007/BF02668372

DO - 10.1007/BF02668372

M3 - Article

SN - 0735-9640

VL - 12

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

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ER -

A simple method for isolation of megabase DNA from cotton

Abstract

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