A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. © 1994 Kluwer Academic Publishers.
|Original language||English (US)|
|Journal||Plant Molecular Biology Reporter|
|State||Published - Jun 1 1994|