A Review of Advanced Molecular Engineering Approaches to Enhance the Thermostability of Enzyme Breakers: From Prospective of Upstream Oil and Gas Industry

Muhammad Naeem, Amjad Bajes Khalil, Zeeshan Tariq, Mohamed Mahmoud

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

During the fracture stimulation of oil and gas wells, fracturing fluids are used to create fractures and transport the proppant into the fractured reservoirs. The fracturing fluid viscosity is responsible for proppant suspension, the viscosity can be increased through the incorporation of guar polymer and cross-linkers. After the fracturing operation, the fluid viscosity is decreased by breakers for efficient oil and gas recovery. Different types of enzyme breakers have been engineered and employed to reduce the fracturing fluid′s viscosity, but thermal stability remains the major con-straint for the use of enzymes. The latest enzyme engineering approaches such as direct evolution and rational design, have great potential to increase the enzyme breakers’ thermostability against high temperatures of reservoirs. In this review article, we have reviewed recently advanced enzyme molecular engineering technologies and how these strategies could be used to enhance the thermo-stability of enzyme breakers in the upstream oil and gas industry.
Original languageEnglish (US)
JournalInternational journal of molecular sciences
Volume23
Issue number3
DOIs
StatePublished - Feb 1 2022
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-20

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Molecular Biology
  • Catalysis
  • Physical and Theoretical Chemistry
  • Inorganic Chemistry
  • Computer Science Applications

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