A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Jianping Zhang, Timothy C. Hewitt, Willem H.P. Boshoff, Ian Dundas, Narayana Upadhyaya, Jianbo Li, Mehran Patpour, Sutha Chandramohan, Zacharias A. Pretorius, Mogens Hovmøller, Wendelin Schnippenkoetter, Robert F. Park, Rohit Mago, Sambasivam Periyannan, Dhara Bhatt, Sami Hoxha, Soma Chakraborty, Ming Luo, Peter Dodds, Burkhard SteuernagelBrande B.H. Wulff, Michael Ayliffe, Robert A. McIntosh, Peng Zhang, Evans S. Lagudah

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.
Original languageEnglish (US)
JournalNature Communications
Volume12
Issue number1
DOIs
StatePublished - Dec 1 2021
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Chemistry
  • General Physics and Astronomy

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