Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.
Bibliographical noteFunding Information:
The authors are indebted to Meritxell Carrió for expert assistance with cell culture techniques. Cristina Menchon is partly supported by a pre-doctoral grant from the Spanish Ministry (AP-2004-3523). This work was partially supported by grants from the Fondo de Investigaciones Sanitarias, Insituto Carlos III (P1071209), MICINN, Fundacion Cellex, CIBER, Sanofi-Aventis and the G. Harold and Leila Y. Mathers Charitable Foundation.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology