A prevalent neglect of environmental control in mammalian cell culture calls for best practices

Shannon Klein, Samhan M. Alsolami, Alexandra Steckbauer, Silvia Arossa, Anieka Parry, Gerardo Ramos Mandujano, Khaled Alsayegh, Juan Carlos Izpisua Belmonte, Mo Li, Carlos M. Duarte

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25 Scopus citations

Abstract

Human cell lines, first cultured in the 1950s1, are indispensable in biomedical research. Today, a wide range of cell types are available, and sophisticated advanced ‘omics’ and visualization techniques allow for the routine assessment of cell identity and cellular responses2. However, the culture methods have remained relatively unchanged. Major advances in culture systems were made over three decades ago3,4, yet the old standard approach of batch cell culture — the culture of cells either in suspension or as adherent monolayers of cells in standard media5,6,7 — remains the predominant method in biomedical research.
Original languageEnglish (US)
JournalNature Biomedical Engineering
DOIs
StatePublished - Aug 13 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-08-16
Acknowledged KAUST grant number(s): BAS/1/1080-01, CRG, OSR, URF/1/3412-01
Acknowledgements: We thank members of the Li laboratory for helpful discussions, and J. Xu and M. K. Y. Sicat for administrative support. We also thank members of the Izpisua Belmonte laboratory for their critical feedback on early versions of the manuscript. This work was supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under award number OSR-2017-CRG-3412. Work in the Izpisua Belmonte laboratory was supported by The Moxie Foundation. The King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) supported the research of the Li laboratory, under award numbers BAS/1/1080-01 and URF/1/3412-01-01. KAUST supported the contribution of the Duarte laboratory through baseline funding to C.M.D.

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