A PLETHORA/PIN-FORMED/auxin network mediates prehaustorium formation in the parasitic plant Striga hermonthica

Ting Ting Xiao; Gwendolyn K. Kirschner; Boubacar A. Kountche; Muhammad Jamil; Maria Savina; Vinicius Lube; Victoria Mironova; Salim Al Babili; Ikram Blilou

doi:10.1093/plphys/kiac215

A PLETHORA/PIN-FORMED/auxin network mediates prehaustorium formation in the parasitic plant Striga hermonthica

Ting Ting Xiao, Gwendolyn K. Kirschner, Boubacar A. Kountche, Muhammad Jamil, Maria Savina, Vinicius Lube, Victoria Mironova, Salim Al Babili, Ikram Blilou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The parasitic plant Striga (Striga hermonthica) invades the host root through the formation of a haustorium and has detrimental impacts on cereal crops. The haustorium results from the prehaustorium, which is derived directly from the differentiation of the Striga radicle. The molecular mechanisms leading to radicle differentiation shortly after germination remain unclear. In this study, we determined the developmental programs that regulate terminal prehaustorium formation in S. hermonthica at cellular resolution. We showed that shortly after germination, cells in the root meristem undergo multiplanar divisions. During growth, the meristematic activity declines and associates with reduced expression of the stem cell regulator PLETHORA1 and the cell cycle genes CYCLINB1 and HISTONE H4. We also observed a basal localization of the PIN-FORMED (PIN) proteins and a decrease in auxin levels in the meristem. Using the structural layout of the root meristem and the polarity of outer-membrane PIN proteins, we constructed a mathematical model of auxin transport that explains the auxin distribution patterns observed during S. hermonthica root growth. Our results reveal a fundamental molecular and cellular framework governing the switch of S.

Original language	English (US)
Pages (from-to)	2281-2297
Number of pages	17
Journal	PLANT PHYSIOLOGY
Volume	189
Issue number	4
DOIs	https://doi.org/10.1093/plphys/kiac215
State	Published - Aug 2022

Bibliographical note

Funding Information:
This study was supported by King Abdullah University of Science and Technology (KAUST) baseline funding given to Ikram Blilou and by the Bill and Melinda Gates Foundation grant OPP1194472 given to Salim Al-Babili. Realistic root models developed in the frame of Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) and the Russian Foundation for Basic Research (RFBR) joint project RFBR-DFG 19-54-12013. Mathematical modeling was supported by FWNR-2022-0020.

Publisher Copyright:
© 2022 American Society of Plant Biologists. All rights reserved.

ASJC Scopus subject areas

Physiology
Genetics
Plant Science

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10.1093/plphys/kiac215

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title = "A PLETHORA/PIN-FORMED/auxin network mediates prehaustorium formation in the parasitic plant Striga hermonthica",

abstract = "The parasitic plant Striga (Striga hermonthica) invades the host root through the formation of a haustorium and has detrimental impacts on cereal crops. The haustorium results from the prehaustorium, which is derived directly from the differentiation of the Striga radicle. The molecular mechanisms leading to radicle differentiation shortly after germination remain unclear. In this study, we determined the developmental programs that regulate terminal prehaustorium formation in S. hermonthica at cellular resolution. We showed that shortly after germination, cells in the root meristem undergo multiplanar divisions. During growth, the meristematic activity declines and associates with reduced expression of the stem cell regulator PLETHORA1 and the cell cycle genes CYCLINB1 and HISTONE H4. We also observed a basal localization of the PIN-FORMED (PIN) proteins and a decrease in auxin levels in the meristem. Using the structural layout of the root meristem and the polarity of outer-membrane PIN proteins, we constructed a mathematical model of auxin transport that explains the auxin distribution patterns observed during S. hermonthica root growth. Our results reveal a fundamental molecular and cellular framework governing the switch of S.",

author = "Xiao, {Ting Ting} and Kirschner, {Gwendolyn K.} and Kountche, {Boubacar A.} and Muhammad Jamil and Maria Savina and Vinicius Lube and Victoria Mironova and {Al Babili}, Salim and Ikram Blilou",

note = "Funding Information: This study was supported by King Abdullah University of Science and Technology (KAUST) baseline funding given to Ikram Blilou and by the Bill and Melinda Gates Foundation grant OPP1194472 given to Salim Al-Babili. Realistic root models developed in the frame of Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) and the Russian Foundation for Basic Research (RFBR) joint project RFBR-DFG 19-54-12013. Mathematical modeling was supported by FWNR-2022-0020. Publisher Copyright: {\textcopyright} 2022 American Society of Plant Biologists. All rights reserved.",

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AU - Xiao, Ting Ting

AU - Kirschner, Gwendolyn K.

AU - Kountche, Boubacar A.

AU - Jamil, Muhammad

AU - Savina, Maria

AU - Lube, Vinicius

AU - Mironova, Victoria

AU - Al Babili, Salim

AU - Blilou, Ikram

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PY - 2022/8

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N2 - The parasitic plant Striga (Striga hermonthica) invades the host root through the formation of a haustorium and has detrimental impacts on cereal crops. The haustorium results from the prehaustorium, which is derived directly from the differentiation of the Striga radicle. The molecular mechanisms leading to radicle differentiation shortly after germination remain unclear. In this study, we determined the developmental programs that regulate terminal prehaustorium formation in S. hermonthica at cellular resolution. We showed that shortly after germination, cells in the root meristem undergo multiplanar divisions. During growth, the meristematic activity declines and associates with reduced expression of the stem cell regulator PLETHORA1 and the cell cycle genes CYCLINB1 and HISTONE H4. We also observed a basal localization of the PIN-FORMED (PIN) proteins and a decrease in auxin levels in the meristem. Using the structural layout of the root meristem and the polarity of outer-membrane PIN proteins, we constructed a mathematical model of auxin transport that explains the auxin distribution patterns observed during S. hermonthica root growth. Our results reveal a fundamental molecular and cellular framework governing the switch of S.

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A PLETHORA/PIN-FORMED/auxin network mediates prehaustorium formation in the parasitic plant Striga hermonthica

Abstract

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