A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Gabriel E. Büchel, Brandon Carney, Jun Tang, Brian M. Zeglis, Jörg Eppinger, Thomas Reiner

Research output: Contribution to journalArticlepeer-review


Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm(2). In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room.
Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number121
StatePublished - Mar 9 2017

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