A novel non-diffractive reconstruction method for digital holographic microscopy

Michael Liebling; Thierry Blu; Etienne Cuche; Pierre Marquet; Christian Depeursinge; Michael Unser

doi:10.1109/ISBI.2002.1029335

A novel non-diffractive reconstruction method for digital holographic microscopy

Michael Liebling, Thierry Blu, Etienne Cuche, Pierre Marquet, Christian Depeursinge, Michael Unser

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

30 Scopus citations

Abstract

We present a new method for reconstructing digitally recorded off-axis Fresnel holograms. Currently-used reconstruction methods are based on the simulation and propagation of a reference wave that is diffracted by the hologram. This procedure introduces a twin-image and a zero-order term which are inherent to the diffraction phenomenon. These terms perturb the reconstruction and limit the field of view. Our new approach splits the reconstruction process into two parts. First, we recover the amplitude and the phase in the camera plane from the measured hologram intensity. Our algorithm is based on the hypothesis of a slowly varying object wave which interferes with a more rapidly varying reference wave. In a second step, we propagate this complex wave to refocus it using the Fresnel transform. We therefore avoid the presence of the twin-image and zero-order interference terms. This new approach is flexible and can be adapted easily to complicated experimental setups. We demonstrate its feasibility in the case of digital holographic microscopy and present results for the imaging of living neurons.

Original language	English (US)
Title of host publication	2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings
Publisher	IEEE Computer Society
Pages	625-628
Number of pages	4
ISBN (Electronic)	078037584X
DOIs	https://doi.org/10.1109/ISBI.2002.1029335
State	Published - 2002
Externally published	Yes
Event	IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States Duration: Jul 7 2002 → Jul 10 2002

Publication series

Name	Proceedings - International Symposium on Biomedical Imaging
Volume	2002-January
ISSN (Print)	1945-7928
ISSN (Electronic)	1945-8452

Other

Other	IEEE International Symposium on Biomedical Imaging, ISBI 2002
Country/Territory	United States
City	Washington
Period	07/7/02 → 07/10/02

Bibliographical note

Publisher Copyright:
© 2002 IEEE.

ASJC Scopus subject areas

Biomedical Engineering
Radiology Nuclear Medicine and imaging

Access to Document

10.1109/ISBI.2002.1029335

Cite this

Liebling, M., Blu, T., Cuche, E., Marquet, P., Depeursinge, C., & Unser, M. (2002). A novel non-diffractive reconstruction method for digital holographic microscopy. In 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings (pp. 625-628). Article 1029335 (Proceedings - International Symposium on Biomedical Imaging; Vol. 2002-January). IEEE Computer Society. https://doi.org/10.1109/ISBI.2002.1029335

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abstract = "We present a new method for reconstructing digitally recorded off-axis Fresnel holograms. Currently-used reconstruction methods are based on the simulation and propagation of a reference wave that is diffracted by the hologram. This procedure introduces a twin-image and a zero-order term which are inherent to the diffraction phenomenon. These terms perturb the reconstruction and limit the field of view. Our new approach splits the reconstruction process into two parts. First, we recover the amplitude and the phase in the camera plane from the measured hologram intensity. Our algorithm is based on the hypothesis of a slowly varying object wave which interferes with a more rapidly varying reference wave. In a second step, we propagate this complex wave to refocus it using the Fresnel transform. We therefore avoid the presence of the twin-image and zero-order interference terms. This new approach is flexible and can be adapted easily to complicated experimental setups. We demonstrate its feasibility in the case of digital holographic microscopy and present results for the imaging of living neurons.",

author = "Michael Liebling and Thierry Blu and Etienne Cuche and Pierre Marquet and Christian Depeursinge and Michael Unser",

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Liebling, M, Blu, T, Cuche, E, Marquet, P, Depeursinge, C & Unser, M 2002, A novel non-diffractive reconstruction method for digital holographic microscopy. in 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings., 1029335, Proceedings - International Symposium on Biomedical Imaging, vol. 2002-January, IEEE Computer Society, pp. 625-628, IEEE International Symposium on Biomedical Imaging, ISBI 2002, Washington, United States, 07/7/02. https://doi.org/10.1109/ISBI.2002.1029335

A novel non-diffractive reconstruction method for digital holographic microscopy. / Liebling, Michael; Blu, Thierry; Cuche, Etienne et al.
2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings. IEEE Computer Society, 2002. p. 625-628 1029335 (Proceedings - International Symposium on Biomedical Imaging; Vol. 2002-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Unser, Michael

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Liebling M, Blu T, Cuche E, Marquet P, Depeursinge C, Unser M. A novel non-diffractive reconstruction method for digital holographic microscopy. In 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings. IEEE Computer Society. 2002. p. 625-628. 1029335. (Proceedings - International Symposium on Biomedical Imaging). doi: 10.1109/ISBI.2002.1029335

A novel non-diffractive reconstruction method for digital holographic microscopy

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