A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries

Simon R. Gerrard, Claire Hardiman, Montserrat Shelbourne, Iris Nandhakumar, Bengt Nordén, Tom Brown

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures. © 2012 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)9221-9228
Number of pages8
JournalACS Nano
Issue number10
StatePublished - Sep 25 2012
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for a grant awarded to B. Norden, and the U.K. BBSRC for the sLoLa grant to T. Brown. "Extending the boundaries of nucleic acid chemistry." Oligonucleotides were synthesised by ATDBio Ltd. We thank Dr. G. John Langley and Julie Herniman for supervision of the oligonucleotide mass spectrometry facility.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.


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