A new equivalent circuit model for micro electroporation systems

Hooman Shagoshtasbi; Yi-Kuen Lee

doi:10.1109/NEMS.2011.6017442

A new equivalent circuit model for micro electroporation systems

Hooman Shagoshtasbi, Yi-Kuen Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Scopus citations

Abstract

Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. © 2011 IEEE.

Original language	English (US)
Title of host publication	2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	662-665
Number of pages	4
ISBN (Print)	9781612847757
DOIs	https://doi.org/10.1109/NEMS.2011.6017442
State	Published - Feb 2011
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Department of Mechanical Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1109/NEMS.2011.6017442

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title = "A new equivalent circuit model for micro electroporation systems",

abstract = "Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. {\textcopyright} 2011 IEEE.",

author = "Hooman Shagoshtasbi and Yi-Kuen Lee",

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AU - Shagoshtasbi, Hooman

AU - Lee, Yi-Kuen

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Department of Mechanical Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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AB - Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. © 2011 IEEE.

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DO - 10.1109/NEMS.2011.6017442

M3 - Conference contribution

SN - 9781612847757

SP - 662

EP - 665

BT - 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

PB - Institute of Electrical and Electronics Engineers (IEEE)

ER -

A new equivalent circuit model for micro electroporation systems

Abstract

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