A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.

Jing Tian; Biao Chen; Bin Zhang; Tantan Li; Zhiqiang Liang; Yujin Guo; Huping Jiao; Fenghong Liang; Longquan Xiang; Fanzhong Lin; Ruiwen Ren; Qingbin Liu

doi:10.1093/labmed/lmac093

A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.

Jing Tian, Biao Chen, Bin Zhang, Tantan Li, Zhiqiang Liang, Yujin Guo, Huping Jiao, Fenghong Liang, Longquan Xiang, Fanzhong Lin, Ruiwen Ren, Qingbin Liu

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Objective: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent. Methods: We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2. Results: By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides within 15 min. The limits of detection and specificity of the auto-RPA-fluorescence platform were 5 copies/µL and 100%, respectively. The accuracy of detection of the auto-RPA-fluorescence platform in the 16 positive samples was 100%. Conclusion: The RPA platform is a potential technology for the diagnosis of SARS-CoV-2 infection.

Original language	English (US)
Journal	Laboratory Medicine
DOIs	https://doi.org/10.1093/labmed/lmac093
State	Published - Oct 6 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-10-10
Acknowledgements: This work was sponsored by the Doctoral Fund of Jining No.1 People’s Hospital (2019004, 2020005), Technology Development Program of Shandong Province (2018WS469), China Postdoctoral Science Foundation (2020T130073ZX), and Natural Science Foundation of Shandong Province (NM ZR2020QH272), China.
We gratefully acknowledge the assistance of Professor Huabao Xiong and Professor Dongmei Shi with the writing of the manuscript.

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1093/labmed/lmac093

https://repository.kaust.edu.sa/bitstream/10754/682310/1/lmac093.pdf

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@article{a463d02ae10c4142962cb3204793f560,

title = "A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.",

abstract = "Objective: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent. Methods: We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2. Results: By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides within 15 min. The limits of detection and specificity of the auto-RPA-fluorescence platform were 5 copies/µL and 100%, respectively. The accuracy of detection of the auto-RPA-fluorescence platform in the 16 positive samples was 100%. Conclusion: The RPA platform is a potential technology for the diagnosis of SARS-CoV-2 infection.",

author = "Jing Tian and Biao Chen and Bin Zhang and Tantan Li and Zhiqiang Liang and Yujin Guo and Huping Jiao and Fenghong Liang and Longquan Xiang and Fanzhong Lin and Ruiwen Ren and Qingbin Liu",

note = "KAUST Repository Item: Exported on 2022-10-10 Acknowledgements: This work was sponsored by the Doctoral Fund of Jining No.1 People{\textquoteright}s Hospital (2019004, 2020005), Technology Development Program of Shandong Province (2018WS469), China Postdoctoral Science Foundation (2020T130073ZX), and Natural Science Foundation of Shandong Province (NM ZR2020QH272), China. We gratefully acknowledge the assistance of Professor Huabao Xiong and Professor Dongmei Shi with the writing of the manuscript.",

year = "2022",

month = oct,

day = "6",

doi = "10.1093/labmed/lmac093",

language = "English (US)",

journal = "Laboratory Medicine",

issn = "0007-5027",

publisher = "Oxford University Press",

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TY - JOUR

T1 - A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.

AU - Tian, Jing

AU - Chen, Biao

AU - Zhang, Bin

AU - Li, Tantan

AU - Liang, Zhiqiang

AU - Guo, Yujin

AU - Jiao, Huping

AU - Liang, Fenghong

AU - Xiang, Longquan

AU - Lin, Fanzhong

AU - Ren, Ruiwen

AU - Liu, Qingbin

N1 - KAUST Repository Item: Exported on 2022-10-10 Acknowledgements: This work was sponsored by the Doctoral Fund of Jining No.1 People’s Hospital (2019004, 2020005), Technology Development Program of Shandong Province (2018WS469), China Postdoctoral Science Foundation (2020T130073ZX), and Natural Science Foundation of Shandong Province (NM ZR2020QH272), China. We gratefully acknowledge the assistance of Professor Huabao Xiong and Professor Dongmei Shi with the writing of the manuscript.

PY - 2022/10/6

Y1 - 2022/10/6

N2 - Objective: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent. Methods: We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2. Results: By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides within 15 min. The limits of detection and specificity of the auto-RPA-fluorescence platform were 5 copies/µL and 100%, respectively. The accuracy of detection of the auto-RPA-fluorescence platform in the 16 positive samples was 100%. Conclusion: The RPA platform is a potential technology for the diagnosis of SARS-CoV-2 infection.

AB - Objective: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent. Methods: We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2. Results: By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides within 15 min. The limits of detection and specificity of the auto-RPA-fluorescence platform were 5 copies/µL and 100%, respectively. The accuracy of detection of the auto-RPA-fluorescence platform in the 16 positive samples was 100%. Conclusion: The RPA platform is a potential technology for the diagnosis of SARS-CoV-2 infection.

UR - http://hdl.handle.net/10754/682310

UR - https://academic.oup.com/labmed/advance-article/doi/10.1093/labmed/lmac093/6749363

U2 - 10.1093/labmed/lmac093

DO - 10.1093/labmed/lmac093

M3 - Article

C2 - 36200614

SN - 0007-5027

JO - Laboratory Medicine

JF - Laboratory Medicine

ER -

A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.

Abstract

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