A microfluidic dialysis device for complex biological mixture SERS analysis

Gerardo Perozziello; Patrizio Candeloro; Francesco T. Gentile; Maria Laura Coluccio; Marco Tallerico; Antonio De Grazia; Annalisa Nicastri; Angela Mena Perri; Elvira Parrotta; Francesca Pardeo; Rossella Catalano; Giovanni Cuda; Enzo M. Di Fabrizio

doi:10.1016/j.mee.2015.02.015

A microfluidic dialysis device for complex biological mixture SERS analysis

Gerardo Perozziello, Patrizio Candeloro, Francesco T. Gentile, Maria Laura Coluccio, Marco Tallerico, Antonio De Grazia, Annalisa Nicastri, Angela Mena Perri, Elvira Parrotta, Francesca Pardeo, Rossella Catalano, Giovanni Cuda, Enzo M. Di Fabrizio

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

Original language	English (US)
Pages (from-to)	37-41
Number of pages	5
Journal	Microelectronic Engineering
Volume	144
DOIs	https://doi.org/10.1016/j.mee.2015.02.015
State	Published - Aug 2015

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01

ASJC Scopus subject areas

Surfaces, Coatings and Films
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Electrical and Electronic Engineering
Condensed Matter Physics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mee.2015.02.015

Cite this

@article{535774f4193f4a18aef24594387a6e25,

title = "A microfluidic dialysis device for complex biological mixture SERS analysis",

abstract = "In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.",

author = "Gerardo Perozziello and Patrizio Candeloro and Gentile, {Francesco T.} and Coluccio, {Maria Laura} and Marco Tallerico and {De Grazia}, Antonio and Annalisa Nicastri and Perri, {Angela Mena} and Elvira Parrotta and Francesca Pardeo and Rossella Catalano and Giovanni Cuda and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01",

year = "2015",

month = aug,

doi = "10.1016/j.mee.2015.02.015",

language = "English (US)",

volume = "144",

pages = "37--41",

journal = "Microelectronic Engineering",

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publisher = "Elsevier B.V.",

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T1 - A microfluidic dialysis device for complex biological mixture SERS analysis

AU - Perozziello, Gerardo

AU - Candeloro, Patrizio

AU - Gentile, Francesco T.

AU - Coluccio, Maria Laura

AU - Tallerico, Marco

AU - De Grazia, Antonio

AU - Nicastri, Annalisa

AU - Perri, Angela Mena

AU - Parrotta, Elvira

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Cuda, Giovanni

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01

PY - 2015/8

Y1 - 2015/8

N2 - In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

AB - In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

UR - http://hdl.handle.net/10754/564194

UR - https://linkinghub.elsevier.com/retrieve/pii/S0167931715000672

UR - http://www.scopus.com/inward/record.url?scp=84924025578&partnerID=8YFLogxK

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DO - 10.1016/j.mee.2015.02.015

M3 - Article

SN - 0167-9317

VL - 144

SP - 37

EP - 41

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

A microfluidic dialysis device for complex biological mixture SERS analysis

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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