A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells

Gerardo Perozziello; Rossella Catalano; Marco Francardi; Eliana Rondanina; Francesca Pardeo; Francesco De De Angelis; Natalia Maria Malara; Patrizio Candeloro; Giovanni Morrone; Enzo M. Di Fabrizio

doi:10.1016/j.mee.2013.02.023

A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells

Gerardo Perozziello, Rossella Catalano, Marco Francardi, Eliana Rondanina, Francesca Pardeo, Francesco De De Angelis, Natalia Maria Malara, Patrizio Candeloro, Giovanni Morrone, Enzo M. Di Fabrizio

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.

Original language	English (US)
Pages (from-to)	314-319
Number of pages	6
Journal	Microelectronic Engineering
Volume	111
DOIs	https://doi.org/10.1016/j.mee.2013.02.023
State	Published - Nov 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (cod.PON01_02782, CUP B71H11000870005), the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University "Magna Graecia" of Catanzaro.

ASJC Scopus subject areas

Surfaces, Coatings and Films
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Electrical and Electronic Engineering
Condensed Matter Physics

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10.1016/j.mee.2013.02.023

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title = "A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells",

abstract = "In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. {\textcopyright} 2013 Elsevier B.V. All rights reserved.",

author = "Gerardo Perozziello and Rossella Catalano and Marco Francardi and Eliana Rondanina and Francesca Pardeo and {De Angelis}, {Francesco De} and Malara, {Natalia Maria} and Patrizio Candeloro and Giovanni Morrone and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the project PON {"}Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti{"} (cod.PON01_02782, CUP B71H11000870005), the project FIRB {"}Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet{"} (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project {"}New Frontiers in Plasmonic Nanosensing{"} (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University {"}Magna Graecia{"} of Catanzaro.",

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doi = "10.1016/j.mee.2013.02.023",

language = "English (US)",

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AU - Perozziello, Gerardo

AU - Catalano, Rossella

AU - Francardi, Marco

AU - Rondanina, Eliana

AU - Pardeo, Francesca

AU - De Angelis, Francesco De

AU - Malara, Natalia Maria

AU - Candeloro, Patrizio

AU - Morrone, Giovanni

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (cod.PON01_02782, CUP B71H11000870005), the project FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" (cod.RBPR05JH2P_010, CUP B41 J09000110005) and Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant No. 2011-0338) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University "Magna Graecia" of Catanzaro.

PY - 2013/11

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N2 - In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.

AB - In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.

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U2 - 10.1016/j.mee.2013.02.023

DO - 10.1016/j.mee.2013.02.023

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JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells

Abstract

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ASJC Scopus subject areas

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