Abstract
We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.
Original language | English (US) |
---|---|
Title of host publication | Methods in molecular biology (Clifton, N.J.) |
Publisher | Springer US |
Pages | 177-188 |
Number of pages | 12 |
ISBN (Print) | 9781493999514 |
DOIs | |
State | Published - Nov 20 2019 |
Bibliographical note
KAUST Repository Item: Exported on 2020-10-01Acknowledged KAUST grant number(s): CRG4
Acknowledgements: This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).