A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector

Zhijian He, Lei Miao, Rainer Jordan, Devika S-Manickam, Robert Luxenhofer, Alexander V. Kabanov

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

© 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Developing safe and efficient non-viral gene delivery systems remains a major challenge. We present a new cationic poly(2-oxazoline) (CPOx) block copolymer for gene therapy that was synthesized by sequential polymerization of non-ionic 2-methyl-2-oxazoline and a new 2-oxazoline monomer, 2-(N-methyl, N-Boc-amino)-methyl-2-oxazoline, followed by deprotection of the pendant secondary amine groups. Upon mixing with plasmid DNA (pDNA), CPOx forms small (diameter ≈80 nm) and narrowly dispersed polyplexes (PDI
Original languageEnglish (US)
Pages (from-to)1004-1020
Number of pages17
JournalMacromolecular Bioscience
Volume15
Issue number7
DOIs
StatePublished - Apr 2 2015
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUK-F1-029-32
Acknowledgements: This study was supported by the Eshelman Gift Trust funds (to A.V.K), by award no. KUK-F1-029-32, made by King Abdullah University of Science and Technology (KAUST), by the Free State of Bavaria, the Fonds der Chemischen Industrie through a junior faculty support grant and start-up funding from the German Plastics Center SKZ and the University of Würzburg (all to R. L.) and partially by the Cancer Nanotechnology Platform Partnership grant (U01 CA116591, to A.V.K) of the National Cancer Institute Alliance for Cancer Nanotechnology. Z. H. is also grateful to GlaxoSmithKline Clinical Research and Drug Development Fellowship support. The authors would like to thank the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) and Proteomics Core Facility at UNC-CH. The authors also acknowledge technical support from Mr. Matthew Haney for the confocal microscopy, Mr. Yuhang Jiang for the gel electrophoresis, Mr. Vivek Mahajan for the supply of pDNA, and Mr. Jonas F. Nawroth for the monomer synthesis.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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