A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector

Zhijian He; Lei Miao; Rainer Jordan; Devika S-Manickam; Robert Luxenhofer; Alexander V. Kabanov

doi:10.1002/mabi.201500021

A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector

Zhijian He, Lei Miao, Rainer Jordan, Devika S-Manickam, Robert Luxenhofer, Alexander V. Kabanov

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

© 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Developing safe and efficient non-viral gene delivery systems remains a major challenge. We present a new cationic poly(2-oxazoline) (CPOx) block copolymer for gene therapy that was synthesized by sequential polymerization of non-ionic 2-methyl-2-oxazoline and a new 2-oxazoline monomer, 2-(N-methyl, N-Boc-amino)-methyl-2-oxazoline, followed by deprotection of the pendant secondary amine groups. Upon mixing with plasmid DNA (pDNA), CPOx forms small (diameter ≈80 nm) and narrowly dispersed polyplexes (PDI

Original language	English (US)
Pages (from-to)	1004-1020
Number of pages	17
Journal	Macromolecular Bioscience
Volume	15
Issue number	7
DOIs	https://doi.org/10.1002/mabi.201500021
State	Published - Apr 2 2015
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUK-F1-029-32
Acknowledgements: This study was supported by the Eshelman Gift Trust funds (to A.V.K), by award no. KUK-F1-029-32, made by King Abdullah University of Science and Technology (KAUST), by the Free State of Bavaria, the Fonds der Chemischen Industrie through a junior faculty support grant and start-up funding from the German Plastics Center SKZ and the University of Würzburg (all to R. L.) and partially by the Cancer Nanotechnology Platform Partnership grant (U01 CA116591, to A.V.K) of the National Cancer Institute Alliance for Cancer Nanotechnology. Z. H. is also grateful to GlaxoSmithKline Clinical Research and Drug Development Fellowship support. The authors would like to thank the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) and Proteomics Core Facility at UNC-CH. The authors also acknowledge technical support from Mr. Matthew Haney for the confocal microscopy, Mr. Yuhang Jiang for the gel electrophoresis, Mr. Vivek Mahajan for the supply of pDNA, and Mr. Jonas F. Nawroth for the monomer synthesis.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1002/mabi.201500021

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title = "A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector",

abstract = "{\textcopyright} 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Developing safe and efficient non-viral gene delivery systems remains a major challenge. We present a new cationic poly(2-oxazoline) (CPOx) block copolymer for gene therapy that was synthesized by sequential polymerization of non-ionic 2-methyl-2-oxazoline and a new 2-oxazoline monomer, 2-(N-methyl, N-Boc-amino)-methyl-2-oxazoline, followed by deprotection of the pendant secondary amine groups. Upon mixing with plasmid DNA (pDNA), CPOx forms small (diameter ≈80 nm) and narrowly dispersed polyplexes (PDI",

author = "Zhijian He and Lei Miao and Rainer Jordan and Devika S-Manickam and Robert Luxenhofer and Kabanov, {Alexander V.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-F1-029-32 Acknowledgements: This study was supported by the Eshelman Gift Trust funds (to A.V.K), by award no. KUK-F1-029-32, made by King Abdullah University of Science and Technology (KAUST), by the Free State of Bavaria, the Fonds der Chemischen Industrie through a junior faculty support grant and start-up funding from the German Plastics Center SKZ and the University of W{\"u}rzburg (all to R. L.) and partially by the Cancer Nanotechnology Platform Partnership grant (U01 CA116591, to A.V.K) of the National Cancer Institute Alliance for Cancer Nanotechnology. Z. H. is also grateful to GlaxoSmithKline Clinical Research and Drug Development Fellowship support. The authors would like to thank the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) and Proteomics Core Facility at UNC-CH. The authors also acknowledge technical support from Mr. Matthew Haney for the confocal microscopy, Mr. Yuhang Jiang for the gel electrophoresis, Mr. Vivek Mahajan for the supply of pDNA, and Mr. Jonas F. Nawroth for the monomer synthesis. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

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T1 - A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector

AU - He, Zhijian

AU - Miao, Lei

AU - Jordan, Rainer

AU - S-Manickam, Devika

AU - Luxenhofer, Robert

AU - Kabanov, Alexander V.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-F1-029-32 Acknowledgements: This study was supported by the Eshelman Gift Trust funds (to A.V.K), by award no. KUK-F1-029-32, made by King Abdullah University of Science and Technology (KAUST), by the Free State of Bavaria, the Fonds der Chemischen Industrie through a junior faculty support grant and start-up funding from the German Plastics Center SKZ and the University of Würzburg (all to R. L.) and partially by the Cancer Nanotechnology Platform Partnership grant (U01 CA116591, to A.V.K) of the National Cancer Institute Alliance for Cancer Nanotechnology. Z. H. is also grateful to GlaxoSmithKline Clinical Research and Drug Development Fellowship support. The authors would like to thank the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) and Proteomics Core Facility at UNC-CH. The authors also acknowledge technical support from Mr. Matthew Haney for the confocal microscopy, Mr. Yuhang Jiang for the gel electrophoresis, Mr. Vivek Mahajan for the supply of pDNA, and Mr. Jonas F. Nawroth for the monomer synthesis. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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N2 - © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Developing safe and efficient non-viral gene delivery systems remains a major challenge. We present a new cationic poly(2-oxazoline) (CPOx) block copolymer for gene therapy that was synthesized by sequential polymerization of non-ionic 2-methyl-2-oxazoline and a new 2-oxazoline monomer, 2-(N-methyl, N-Boc-amino)-methyl-2-oxazoline, followed by deprotection of the pendant secondary amine groups. Upon mixing with plasmid DNA (pDNA), CPOx forms small (diameter ≈80 nm) and narrowly dispersed polyplexes (PDI

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JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

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ER -

A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector

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